PMID: 9420236Jan 7, 1998Paper

Infectious transcripts from cloned genome-length cDNA of porcine reproductive and respiratory syndrome virus

Journal of Virology
J J MeulenbergR J Moormann

Abstract

The 5'-terminal end of the genomic RNA of the Lelystad virus isolate (LV) of porcine reproductive and respiratory syndrome virus was determined. To construct full-length cDNA clones, the 5'-terminal sequence was ligated to cDNA clones covering the complete genome of LV. When RNA that was transcribed in vitro from these full-length cDNA clones was transfected into BHK-21 cells, infectious LV was produced and secreted. The virus was rescued by passage to porcine alveolar lung macrophages or CL2621 cells. When infectious transcripts were transfected to porcine alveolar lung macrophages or CL2621 cells, no infectious virus was produced due to the poor transfection efficiency of these cells. The growth properties of the viruses produced by BHK-21 cells transfected with infectious transcripts of LV cDNA resembled the growth properties of the parental virus from which the cDNA was derived. Two nucleotide changes leading to a unique PacI restriction site directly downstream of the ORF7 gene were introduced in the genome-length cDNA clone. The virus recovered from this mutated cDNA clone retained the PacI site, which confirmed the de novo generation of infectious LV from cloned cDNA. These results indicate that the infectious clone of L...Continue Reading

References

Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J E CollinsH S Joo
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D A BenfieldD Chladek
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G WensvoortD Chladek
Jul 1, 1991·The Veterinary Quarterly·G WensvoortF Wagenaar
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C J LaiM Bray
Dec 1, 1988·The Journal of General Virology·W SpaanM C Horzinek
Jan 10, 1995·Virology·J J MeulenbergG Wensvoort
Nov 1, 1993·The Journal of General Virology·E J Snijder, M C Horzinek
Feb 1, 1994·Virology·J C Boyer, A L Haenni
Aug 1, 1993·The Journal of General Virology·J J MeulenbergR J Moormann
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·L C van DintenE J Snijder

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Citations

May 12, 2007·Journal of Virology·Jay G CalvertSiao-Kun W Welch
Mar 22, 2000·The Journal of General Virology·J ZiebuhrA E Gorbalenya
Oct 17, 2001·The Journal of General Virology·M H VerheijeJ J Meulenberg
Apr 23, 2010·The Journal of General Virology·Wander Van BreedamHans J Nauwynck
Sep 16, 2011·The Journal of General Virology·Tayyba T Baig, Alexander Zakhartchouk
Aug 14, 2013·The Journal of General Virology·Eric J SnijderYing Fang
Dec 13, 2002·Journal of Virology·Eric J SnijderWilly J M Spaan
Nov 15, 2008·The Journal of General Virology·Hanne Van GorpHans J Nauwynck
Feb 5, 2008·Science in China. Series C, Life Sciences·ShiShan Yuan, ZuZhang Wei
Jul 8, 2014·Pharmaceutical Patent Analyst·Kathleen L Hefferon
Mar 8, 2018·Current Protocols in Microbiology·Zexu MaYan-Jin Zhang
May 13, 2006·The Journal of General Virology·Alexander O PasternakEric J Snijder
Feb 17, 2018·Archives of Virology·Yifeng QinWeijian Huang
Mar 22, 2007·The Journal of General Virology·Erwin van den BornEric J Snijder
Dec 19, 2000·Virology·M H Groot Bramel-VerheijeJ J Meulenberg

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