Infective and inactivated filamentous phage as carriers for immunogenic peptides

Journal of Virological Methods
Tatiana I SamoylovaNancy R Cox

Abstract

The focus of this study is on development of vaccines using filamentous phage as a delivery vector for immunogenic peptides. The use of phage as a carrier for immunogenic peptides provides significant benefits such as high immunogenicity, low production costs, and high stability of phage preparations. However, introduction of live recombinant phage into the environment might represent a potential ecological problem. This, for example, may occur when vaccines are used in oral or nasal formulations in field conditions for wild and feral animals. To address this issue, comparative studies of antigenic properties of live and inactivated (non-viable) phage were accomplished. Inactivated phage, if released, will not propagate and will degrade as any other protein. In these experiments, a model phage clone that was previously selected from a phage display library and shown to stimulate production of anti-sperm antibodies with contraceptive properties was used. Multiple methods of phage inactivation were tested, including drying, freezing, autoclaving, heating, and UV irradiation. Under studied conditions, heating at 76°C for 3h, UV irradiation, and autoclaving resulted in complete phage inactivation. Phage samples treated by heat and ...Continue Reading

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Citations

Apr 5, 2013·Journal of Clinical Microbiology·Jennifer D RogersTimothy Palzkill
Jul 24, 2004·Journal of Bioinformatics and Computational Biology·Werner G Krebs, Philip E Bourne
Jan 25, 2007·Journal of Bioinformatics and Computational Biology·Gek-Huey ChuaMasaru Tomita
May 5, 2007·Journal of Bioinformatics and Computational Biology·Troy Hawkins, Daisuke Kihara
Sep 21, 2013·Drug Discovery Today·Maryam Hamzeh-MivehroudSiavoush Dastmalchi
Nov 23, 2017·Frontiers in Microbiology·Anna KłopotKrystyna Dąbrowska
May 17, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jie LiRongfei Zhu
Jul 15, 2020·Parasitology·Marianna Nascimento ManhaniJulia Maria Costa-Cruz
Apr 25, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oleg Krut, Isabelle Bekeredjian-Ding
Sep 10, 2020·Vaccines·Alejandro González-MoraJorge Benavides
Mar 7, 2021·Pharmaceuticals·Valentina Foglizzo, Serena Marchiò

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