PMID: 3300131Jan 1, 1987

Infectivity of influenza B virus in cultured human muscle

Acta Neuropathologica
S ServideiE T Gamboa

Abstract

Severe muscle symptoms, particularly in children, are frequently associated with influenza B virus infection. In this study we examined the effects of influenza B virus (Lee Strain) on cultured human muscle by light and electron microscopy (EM), immunofluorescence, hemadsorption and plaque assays. Muscle injury was also evaluated by the appearance of muscle-specific creatine kinase (CK) in the culture medium. By fluorescence immunocytochemistry viral antigen was demonstrated in muscle cell nuclei within 3 h postinoculation (p.i.) and in the cytoplasm at 6 h p.i. Membrane-associated viral antigen was seen at 16 h p.i., at which time budding influenza virus-like particles could be demonstrated by EM, both in myoblasts and multinucleated myotubes. At 16 h all cells were hemadsorption positive. Plaque assays showed peak virus production at 48 h (p.i.), at which time cytopathic effects (cell retraction, pycnosis and cytoplasmic vacuolization) were prominent and some cells detached from the substratum. Leakage of muscle-specific CK isozyme into the culture medium could be demonstrated as early as 6 h p.i. with peak enzyme activity around 40-48 h p.i. Cytopathic changes and virus production were observed both in myoblasts and myotubes...Continue Reading

References

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Citations

Oct 1, 1977·The Journal of General Virology·N J CoxA P Kendal
Feb 1, 1977·Annals of Internal Medicine·T P GrecoM Kashgarian
Sep 12, 1970·Lancet·P J MiddletonM T Szymanski
Sep 1, 1970·Biochemical Pharmacology·L T Kremzner, D H Harter
Mar 25, 1974·JAMA : the Journal of the American Medical Association·B S BerlinR N Bovner
Jan 1, 1974·Archiv für die gesamte Virusforschung·D H HarterJ E Coward
Jan 1, 1973·Tissue & Cell·A F Miranda, G C Godman
Dec 1, 1973·Archives of Neurology·J D MejlszenkierE M Ouellette
Mar 1, 1974·Annals of Internal Medicine·R A MinowL Dornfeld
Jan 1, 1983·Annual Review of Biochemistry·R A Lamb, P W Choppin
Nov 1, 1984·Virology·M W Shaw, P W Choppin
Jan 1, 1984·Journal of Neurology·M R Higatsberger, E Auff
Nov 15, 1982·European Journal of Biochemistry·L D BergelsonE V Fomina-Ageeva
Aug 1, 1980·Neurology·F CongyR Moulias
Feb 1, 1980·JAMA : the Journal of the American Medical Association·H A KesslerS Levin
Jan 1, 1957·Acta Paediatrica·A LUNDBERG

Related Concepts

Myoblasts
Immunofluorescence Assay
Virus Diseases
Macro-Creatine Kinase
AS 6
Extravasation
PDZK1IP1
Virus Replication
Influenza
Immunocytochemistry

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