PMID: 3300131Jan 1, 1987

Infectivity of influenza B virus in cultured human muscle

Acta Neuropathologica
S ServideiE T Gamboa


Severe muscle symptoms, particularly in children, are frequently associated with influenza B virus infection. In this study we examined the effects of influenza B virus (Lee Strain) on cultured human muscle by light and electron microscopy (EM), immunofluorescence, hemadsorption and plaque assays. Muscle injury was also evaluated by the appearance of muscle-specific creatine kinase (CK) in the culture medium. By fluorescence immunocytochemistry viral antigen was demonstrated in muscle cell nuclei within 3 h postinoculation (p.i.) and in the cytoplasm at 6 h p.i. Membrane-associated viral antigen was seen at 16 h p.i., at which time budding influenza virus-like particles could be demonstrated by EM, both in myoblasts and multinucleated myotubes. At 16 h all cells were hemadsorption positive. Plaque assays showed peak virus production at 48 h (p.i.), at which time cytopathic effects (cell retraction, pycnosis and cytoplasmic vacuolization) were prominent and some cells detached from the substratum. Leakage of muscle-specific CK isozyme into the culture medium could be demonstrated as early as 6 h p.i. with peak enzyme activity around 40-48 h p.i. Cytopathic changes and virus production were observed both in myoblasts and myotubes...Continue Reading


Jul 7, 2001·Journal of the Neurological Sciences·L E Davis, M Kornfeld
Nov 19, 2002·Pediatric Neurology·Tatsuo MasuyamaYuhei Hamasaki
Feb 1, 2012·The Journal of Infectious Diseases·Christopher D PaddockSherif R Zaki
Jun 4, 2008·The Pediatric Infectious Disease Journal·Chia-Yu ChiChing-Chuan Liu
Jul 16, 2008·Clinical Microbiology Reviews·Nancy F Crum-Cianflone
Apr 3, 2013·Journal of Neurosciences in Rural Practice·Summer Bell GibsonMark B Bromberg
Mar 10, 2010·European Journal of Pediatrics·Esther RubínCristina Flores
Feb 6, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M ParikhW Sangsiraprapha
Mar 18, 2006·Rheumatology·M GomesU A Walker
Oct 24, 2020·European Journal of Pediatrics·James KerrPhilip N Britton


Oct 1, 1977·The Journal of General Virology·N J CoxA P Kendal
Feb 1, 1977·Annals of Internal Medicine·T P GrecoM Kashgarian
Sep 12, 1970·Lancet·P J MiddletonM T Szymanski
Sep 1, 1970·Biochemical Pharmacology·L T Kremzner, D H Harter
Mar 25, 1974·JAMA : the Journal of the American Medical Association·B S BerlinR N Bovner
Jan 1, 1974·Archiv für die gesamte Virusforschung·D H HarterJ E Coward
Jan 1, 1973·Tissue & Cell·A F Miranda, G C Godman
Dec 1, 1973·Archives of Neurology·J D MejlszenkierE M Ouellette
Mar 1, 1974·Annals of Internal Medicine·R A MinowL Dornfeld
Jan 1, 1983·Annual Review of Biochemistry·R A Lamb, P W Choppin
Nov 1, 1984·Virology·M W Shaw, P W Choppin
Jan 1, 1984·Journal of Neurology·M R Higatsberger, E Auff
Nov 15, 1982·European Journal of Biochemistry·L D BergelsonE V Fomina-Ageeva
Aug 1, 1980·Neurology·F CongyR Moulias
Feb 1, 1980·JAMA : the Journal of the American Medical Association·H A KesslerS Levin
Jan 1, 1957·Acta Paediatrica·A LUNDBERG

Related Concepts

Immunofluorescence Assay
Virus Diseases
Macro-Creatine Kinase
AS 6
Virus Replication

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.