PMID: 13575680Oct 1, 1958Paper

Infectivity of ribonucleic acid from poliovirus in human cell monolayers

The Journal of Experimental Medicine
H E ALEXANDERO VAN DAMME

Abstract

Ribonucleic acid prepared by the method of Gierer and Schramm from concentrated and partially purified types I and II polioviruses has been demonstrated to be infectious for HeLa and human amnion cells in monolayers. In areas of cytopathogenic action resulting from invasion of cells by RNA, intact poliovirus, of the type from which the RNA had been prepared, is present. The infectivity of the RNA was completely inactivated by a 2 minute exposure to purified ribonuclease or to whole normal monkey serum shown to contain measurable concentrations of this enzyme. Whole virus infectivity was not influenced by RNAase or whole normal monkey serum. Normal and polio-immune globulin, desoxyribonuclease, lysozyme, proteolytic enzymes, and bovine albumin failed to inactivate the infectivity of RNA. The degree of infectivity of isolated RNA from poliovirus for cells in monolayer was greatly influenced by the ionic strength of the environment. The experimental evidence suggests that isolated poliovirus RNA is the carrier of the biological activity responsible for infection of cells and for transmission of genetic information which controls type specificity.

References

Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·I M MOUNTAIN
Apr 14, 1956·Nature·A GIERER, G SCHRAMM
Oct 1, 1956·Virology·F L SCHAFFER, C E SCHWERDT
Mar 1, 1957·Proceedings of the Society for Experimental Biology and Medicine·J FOGH, R O LUND
Feb 1, 1958·Virology·H E ALEXANDERO VAN DAMME

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Citations

Jun 7, 1968·Acta Neuropathologica·C H SuttonH M Zimmerman
Sep 9, 1964·Archiv für die gesamte Virusforschung·H ROUHANDEH
Jan 1, 1971·Archiv für die gesamte Virusforschung·F A RichterN A Labzoffsky
Jan 1, 1964·Archiv für die gesamte Virusforschung·F HORODNICEANUA A COMBIESCU
Dec 1, 1968·The Journal of Hygiene·J C de Jong, K C Winkler
Mar 11, 1961·Nature·R M HERRIOTTS GUPTA
Dec 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·G A DimayorcaA Bendich
Jun 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·H F Maassab
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·D H MOOREE C POLLARD
Jan 1, 1961·The Journal of Biophysical and Biochemical Cytology·E Borenfreund, A Bendich
May 1, 1959·The Journal of Experimental Medicine·J J HOLLAND, L C McLAREN
Apr 1, 1960·The Journal of Experimental Medicine·P GERBER, R KIRSCHSTEIN
Nov 1, 1960·The Journal of Experimental Medicine·J J HOLLANDJ T SYVERTON
Nov 1, 1960·The Journal of Experimental Medicine·J J HOLLANDJ T SYVERTON
Apr 30, 1960·British Medical Journal
Apr 1, 1964·The Journal of Clinical Investigation·J A MANNICK
Mar 1, 1961·Virology·J E DARNELLJ L HOOPER
Aug 1, 1960·Virology·J E DARNELL, T K SAWYER
May 1, 1996·The Journal of Pediatrics·M Katz
Jun 5, 2015·PLoS Pathogens·Nicolas Lévêque, Bert L Semler
Aug 1, 1964·Virology·D L ENGELHARDT, N D ZINDER

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