Infectivity phenotypes of H3N2 influenza A viruses in primary swine respiratory epithelial cells are controlled by sialic acid binding.

Influenza and Other Respiratory Viruses
Allen C BatemanC W Olsen

Abstract

In the late 1990s, triple reassortant H3N2 influenza A viruses emerged and spread widely in the US swine population. We have shown previously that an isolate representative of this virus-lineage, A/Swine/Minnesota/593/99 (Sw/MN), exhibits phenotypic differences compared to a wholly human-lineage H3N2 virus isolated during the same time period, A/Swine/Ontario/00130/97 (Sw/ONT). Specifically, Sw/MN was more infectious for pigs and infected a significantly higher proportion of cultured primary swine respiratory epithelial cells (SRECs). In addition, reverse genetics-generated Sw/MN × Sw/ONT reassortant and point mutant viruses demonstrated that the infectivity phenotypes in SRECs were strongly dependent on three amino acids within the hemagglutinin (HA) gene. To determine the mechanism by which Sw/MN attains higher infectivity than Sw/ONT in SRECs. A/Swine/Minnesota/593/99, Sw/ONT, and mutant (reverse genetics-generated HA reassortant and point mutant) viruses were compared at various HA-mediated stages of infection: initial sialic acid binding, virus entry, and the pH of virus-endosome fusion. Sialic acid binding was the sole stage where virus differences directly paralleled infectivity phenotypes in SRECs, indicating that bindi...Continue Reading

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Citations

Mar 1, 2013·British Poultry Science·Ilaria Capua
Jun 4, 2013·Virus Research·Mariana BazKanta Subbarao
Mar 7, 2013·Influenza and Other Respiratory Viruses·Beverley J PatersonKate Hardie
May 25, 2013·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Dena L Schanzer, Brian Schwartz
Aug 19, 2014·Journal of Medical Virology·Kelsey M HenriquezBruce Barrett
Feb 2, 2013·The Oncologist·Leslie S KersunKathleen E Sullivan
Apr 19, 2013·Clinical and Experimental Vaccine Research·Dong-Hun Lee, Chang-Seon Song

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