Inference about causation between body mass index and DNA methylation in blood from a twin family study

International Journal of Obesity : Journal of the International Association for the Study of Obesity
Shuai LiJohn L Hopper

Abstract

Several studies have reported DNA methylation in blood to be associated with body mass index (BMI), but few have investigated causal aspects of the association. We used a twin family design to assess this association at two life points and applied a novel analytical approach to appraise the evidence for causality. The methylation profile of DNA from peripheral blood was measured for 479 Australian women from 130 twin families. Linear regression was used to estimate the associations of DNA methylation at ~410,000 cytosine-guanine dinucleotides (CpGs), and of the average DNA methylation at ~20,000 genes, with current BMI, BMI at age 18-21 years, and the change between the two (BMI change). A novel regression-based methodology for twins, Inference about Causation through Examination of Familial Confounding (ICE FALCON), was used to assess causation. At a 5% false discovery rate, nine, six and 12 CpGs at 24 loci were associated with current BMI, BMI at age 18-21 years and BMI change, respectively. The average DNA methylation of the BHLHE40 and SOCS3 loci was associated with current BMI, and of the PHGDH locus with BMI change. From the ICE FALCON analyses with BMI as the predictor and DNA methylation as the outcome, a woman's DNA me...Continue Reading

References

Dec 21, 2002·European Journal of Biochemistry·Megumi MasakiToshisuke Kawasaki
Oct 6, 2005·The Journal of Biological Chemistry·Christine McDonaldGabriel Nuñez
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mihai G NeteaSoo-Hyun Kim
Jan 18, 2006·Microvascular Research·Michael P DaveyJames T Rosenbaum
Feb 14, 2006·The American Journal of Clinical Nutrition·Andrew S Greenberg, Martin S Obin
Apr 25, 2006·Biostatistics·W Evan JohnsonAriel Rabinovic
Mar 14, 2008·The New England Journal of Medicine·Manel Esteller
Dec 10, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Gillian S DiteJohn L Hopper
Feb 11, 2010·Cancer Research·Fabrice OdefreyUNKNOWN Australian Twins and Sisters Mammographic Density Study
Aug 14, 2010·Mediators of Inflammation·Rosário Monteiro, Isabel Azevedo
Dec 24, 2010·BMC Medicine·Xiaoling WangHuidong Shi
Jan 12, 2011·Annual Review of Immunology·Margaret F Gregor, Gökhan S Hotamisligil
Sep 3, 2011·Inflammatory Bowel Diseases·Yun ZhaoHans-Christian Reinecker
Dec 21, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Madhumouli ChatterjeeGeorge C Tsokos
Apr 28, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jennifer StoneJohn L Hopper
May 10, 2012·BMC Bioinformatics·Eugene Andres HousemanKarl T Kelsey
Oct 2, 2012·The Journal of Allergy and Clinical Immunology·John L HopperShyamali C Dharmage
Dec 12, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jordi SintesPablo Engel
Mar 19, 2013·BMC Biotechnology·JiHoon E JooMelissa C Southey
Mar 19, 2014·Lancet·Katherine J DickNilesh J Samani
Apr 1, 2014·Epidemiology·Tyler J VanderWeelePeter Kraft
Apr 17, 2014·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Shaoyong SuXiaoling Wang
Nov 4, 2015·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Shuai LiJohn L Hopper
Jan 30, 2016·Clinical Epigenetics·Wadha A Al MuftahKarsten Suhre
Jul 22, 2016·Human Molecular Genetics·R C RichmondC L Relton
Aug 27, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Omar AliYi Zhang
Oct 25, 2016·International Journal of Obesity : Journal of the International Association for the Study of Obesity·L E WilsonJ A Taylor
Feb 28, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S LiJ L Hopper
Dec 27, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Y M GeurtsR L Milne

❮ Previous
Next ❯

Citations

Jan 28, 2020·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Jeffrey M CraigDedra Buchwald
Sep 13, 2019·Circulation Research·Dianjianyi SunWei Chen
Nov 27, 2019·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Kate MurphyJohn L Hopper
Sep 10, 2020·Epigenomics·Weilong LiQihua Tan
Apr 17, 2020·Molecular Diagnosis & Therapy·Qihua Tan
Dec 6, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Ida K KarlssonAnna K Dahl Aslan
Apr 17, 2021·Environment International·Rongbin XuYuming Guo
Dec 15, 2020·Seminars in Cell & Developmental Biology·Amita G GhadgeWendy V Ingman
Jul 20, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Whitney L DoK M Venkat Narayan

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE100227

Methods Mentioned

BETA
ICE

Software Mentioned

FALCON
Bioconductor minfi package
ComBat
ICE FALCON
R package geepack

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.