Inferior Colliculus Transcriptome After Status Epilepticus in the Genetically Audiogenic Seizure-Prone Hamster GASH/Sal

Frontiers in Neuroscience
Sandra M Díaz-RodríguezDolores E Lopéz

Abstract

The Genetic Audiogenic Seizure Hamster from Salamanca (GASH/Sal), an animal model of reflex epilepsy, exhibits generalized tonic-clonic seizures in response to loud sound with the epileptogenic focus localized in the inferior colliculus (IC). Ictal events in seizure-prone strains cause gene deregulation in the epileptogenic focus, which can provide insights into the epileptogenic mechanisms. Thus, the present study aimed to determine the expression profile of key genes in the IC of the GASH/Sal after the status epilepticus. For such purpose, we used RNA-Seq to perform a comparative study between the IC transcriptome of GASH/Sal and that of control hamsters both subjected to loud sound stimulation. After filtering for normalization and gene selection, a total of 36 genes were declared differentially expressed from the RNA-seq analysis in the IC. A set of differentially expressed genes were validated by RT-qPCR showing significant differentially expression between GASH/Sal hamsters and Syrian control hamsters. The confirmed differentially expressed genes were classified on ontological categories associated with epileptogenic events similar to those produced by generalized tonic seizures in humans. Subsequently, based on the resul...Continue Reading

References

Jan 1, 1986·Annual Review of Immunology·H J Müller-Eberhard
Apr 5, 1974·Brain Research·M Tachibana, K Kuriyama
Jun 1, 1966·Experimental Neurology·R P Kesner
Aug 1, 1970·Experimental Neurology·J A WadaE Jung
Nov 1, 1982·Molecular Immunology·R G Discipio, J Gagnon
Feb 1, 1994·The Journal of Comparative Neurology·D L OliverR L Saint Marie
Mar 1, 1996·Pharmacology, Biochemistry, and Behavior·N Garcia-CairascoK O Nonaka
Oct 6, 1997·Neurochemistry International·A M Beckmann, P A Wilce
Nov 18, 1998·Journal of Neurophysiology·D R MooreD H Sanes
Aug 15, 2000·Neuroscience and Biobehavioral Reviews·K C Ross, J R Coleman
Aug 30, 2000·Hearing Research·F C Richardson, L K Kaczmarek
Apr 5, 2001·The New England Journal of Medicine·M J Walport
Mar 26, 2003·Clinical and Experimental Immunology·M A Dragon-DureyM D Kazatchkine
Dec 11, 2003·Journal of Pediatric Surgery·Hideki TomiyamaAlexander I Agoulnik
Nov 27, 2004·Journal of Cell Science·Jochen HessMarina Schorpp-Kistner
Jan 15, 2005·Virus Research·Marçal Pastor-AngladaF Javier Casado
Jun 29, 2005·Brain Research Bulletin·Xianchun LiYinghe Hu
Aug 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·D S RobertsS J Russek
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Sep 26, 2008·Nature·Yingxi LinMichael E Greenberg
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Dec 23, 2008·Cancer Genetics and Cytogenetics·Branka KorosecMetka Ravnik-Glavac
Nov 20, 2009·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Ole B SuhrPer Westermark
Jun 23, 2011·PloS One·Kellen D WindenJerome Engel
Nov 8, 2011·Molecular Cell·Trent FowlerAnanda L Roy
Nov 15, 2011·Molecular Genetics and Metabolism·Bikash R PattnaikDe-Ann M Pillers
Nov 19, 2011·Lancet Neurology·Violaine Planté-Bordeneuve, Gerard Said

❮ Previous
Next ❯

Citations

Feb 9, 2021·Frontiers in Behavioral Neuroscience·Giselda Cabral-PereiraRicardo Gómez-Nieto

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-Seq
PCR
cDNA library construction

Software Mentioned

STAR
EdgeR
R
STRING
GASH
SD
Ensembl
Sal
FASTQC
PANTHER

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.