Apr 3, 2020

Versatile workflow for cell type resolved transcriptional and epigenetic profiles from cryopreserved human lung.

BioRxiv : the Preprint Server for Biology
M. Llamazares PradaRenata Zofia Jurkowska


The complexity of the lung microenvironment together with changes in cellular composition during disease progression make it exceptionally hard to understand the molecular mechanisms leading to the development of chronic lung diseases. Although recent advances in cell type resolved and single-cell sequencing approaches hold great promise for studying complex diseases, their implementation greatly relies on local access to fresh tissue, as traditional methods to process and store tissue do not allow viable cell isolation. To overcome these hurdles, we developed a novel, versatile workflow that allows long-term storage of human lung tissue with high cell viability, permits thorough sample quality check before cell isolation, and is compatible with next generation sequencing-based profiling, including single-cell approaches. We demonstrate that cryopreservation is suitable for isolation of multiple cell types from different lung locations and is applicable to both healthy and diseased tissue, including COPD and tumor samples. Basal cells isolated from cryopreserved airways retain the ability to differentiate, indicating that cellular identity is not altered by cryopreservation. Importantly, using RNA sequencing (RNA-seq) and Illum...Continue Reading

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Extracellular Matrix
Anatomical Space Structure

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

The Journal of Burn Care & Rehabilitation
C C Bondoc
Molecular and Cellular Biochemistry
Hiroki YokomuroRen-Ke Li
The International Journal of Artificial Organs
F Arnaud
Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
Feng XuUtkan Demirci
© 2020 Meta ULC. All rights reserved