Infertility induced by auxin in PX627 Caenorhabditis elegans does not affect mitochondrial functions and aging parameters.

Aging
B. DilbergerGunter P Eckert

Abstract

Caenorhabditis elegans is widely used for aging studies. 5-Fluoro-2´-deoxyuridine (FUdR) is commonly used to control offspring. While larvae are stopped from further development, also mitochondrial DNA and function may be affected. Since mitochondria and longevity are closely related, the use of FUdR may falsify possible studies. PX627, an auxin inducible infertility strain to control offspring, allows mitochondrial investigations during senescence without FUdR toxicity.Longevity and health parameters were assessed in 2- and 10-day old nematodes wild-type N2 and PX627 treated with FUdR or auxin, respectively. Mitochondrial membrane potential, energetic metabolites and reactive oxygen species levels, were determined. mRNA expression levels of key genes involved were quantified using quantitative real-time PCR.FUdR significantly increased lifespan and health parameters, as well as, mitochondrial function compared to untreated controls and auxin treated PX627. Although a decrease in all parameters could be observed in aged nematodes, this was less severe after FUdR exposure. Glycolysis was significantly up-regulated in aged PX627 compared to N2. Expression levels of daf-16, sir-2.1, aak-2, skn-1, atp-2 and atfs-1 were regulated ac...Continue Reading

References

Dec 5, 1974·Journal of Molecular Biology·R H WaterstonS Brenner
May 1, 1974·Genetics·S Brenner
Dec 31, 2003·Experimental Gerontology·Thomas E Johnson
Feb 18, 2004·Aging Cell·David G Nicholls
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Cornelia I Bargmann
Sep 7, 2011·Mechanisms of Ageing and Development·Jeremy Michael Van Raamsdonk, Siegfried Hekimi
Mar 31, 2012·Lab on a Chip·Pouya RezaiBhagwati P Gupta
Jul 24, 2012·Biochemistry. Biokhimii︠a︡·D A Knorre, F F Severin
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Andreas H LudewigFrank C Schroeder
May 7, 2013·Journal of Visualized Experiments : JoVE·Olivia MargieIan Chin-Sang
Jan 17, 2014·Nature·Jonathan R Friedman, Jodi Nunnari
Apr 8, 2014·Experimental Gerontology·J P RooneyJ N Meyer
Apr 22, 2015·Nature·Konstantinos PalikarasNektarios Tavernarakis
Feb 9, 2016·Mechanisms of Ageing and Development·Edward N AndersonAnne C Hart
Aug 31, 2016·Critical Reviews in Food Science and Nutrition·Peiyi ShenYeonhwa Park
Feb 28, 2017·Molecules and Cells·Hae-Eun H ParkSeung-Jae V Lee
Aug 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Robert SonowalDaniel Kalman
Oct 11, 2017·Journal of Experimental Botany·Elizabeth M Frick, Lucia C Strader
Oct 17, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bryan A MartinezKim A Caldwell
Oct 19, 2017·Human Reproduction·Yifan LiuYuanqing Yao
Nov 9, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sumino YanaseNaoaki Ishii
Feb 17, 2018·Journal of Neuroscience Methods·Michele PerniTuomas P J Knowles
Jun 9, 2018·G3 : Genes - Genomes - Genetics·Katja R KasimatisPatrick C Phillips
Aug 24, 2018·ACS Chemical Biology·Mussa QuareshyRichard M Napier
Jan 7, 2019·Biochemical and Biophysical Research Communications·Hongyuan WangZhizhou Zhang
Mar 29, 2019·Advances in Experimental Medicine and Biology·Sumino YanaseNaoaki Ishii
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Benjamin DilbergerGunter P Eckert

❮ Previous
Next ❯

Citations

Jan 27, 2021·Biology Open·Anupam BhoiPaola Fabrizio
Jun 18, 2021·Mechanisms of Ageing and Development·Benjamin DilbergerGunter P Eckert

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electron microscopy
transgenic
Protein Assay

Software Mentioned

Prism
DatLab
ImageJ
WF NTP ( Field - of - view Nematode Tracking Platform )
GraphPad
FUdR

Related Concepts

Related Feeds

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Aging Genetics (Keystone)

This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.