Infiltration of Matrix-Non-producers Weakens the Salmonella Biofilm and Impairs Its Antimicrobial Tolerance and Pathogenicity

Frontiers in Microbiology
Chakravarthy S SrinandanDipshikha Chakravortty

Abstract

Bacterial biofilms display a collective lifestyle, wherein the cells secrete extracellular polymeric substances (EPS) that helps in adhesion, aggregation, stability, and to protect the bacteria from antimicrobials. We asked whether the EPS could act as a public good for the biofilm and observed that infiltration of cells that do not produce matrix components weakened the biofilm of Salmonella enterica serovar Typhimurium. EPS production was costly for the producing cells, as indicated by a significant reduction in the fitness of wild type (WT) cells during competitive planktonic growth relative to the non-producers. Infiltration frequency of non-producers in the biofilm showed a concomitant decrease in overall productivity. It was apparent in the confocal images that the non-producing cells benefit from the EPS produced by the Wild Type (WT) to stay in the biofilm. The biofilm containing non-producing cells were more significantly susceptible to sodium hypochlorite and ciprofloxacin treatment than the WT biofilm. Biofilm infiltrated with non-producers delayed the pathogenesis, as tested in a murine model. The cell types were spatially assorted, with non-producers being edged out in the biofilm. However, cellulose was found to a...Continue Reading

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Citations

Jul 28, 2016·Frontiers in Microbiology·Zhiyan HeJingping Liang
Jan 17, 2017·Trends in Microbiology·Anna Dragoš, Ákos T Kovács
Sep 1, 2017·International Journal of Molecular Sciences·Enea Gino Di DomenicoFabrizio Ensoli
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Oct 20, 2018·PLoS Pathogens·Jurgen WuytsMichelle Holtappels
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Nov 27, 2020·FEMS Microbiology Ecology·Sudipa Maity, Kiran Ambatipudi
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Oct 16, 2020·FEMS Microbiology Ecology·Sandeep MiryalaC S Srinandan
Nov 3, 2017·ACS Applied Materials & Interfaces·Yadavali Siva PrasadSubbiah Nagarajan

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Methods Mentioned

BETA
PCR
microsensors

Software Mentioned

COMSTAT
ADOBE Photoshop®
Imaris
MATLAB
DAIME

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