Infiltration of nestin-expressing cells in interstitial fibrosis in chronic cyclosporine nephropathy

Transplantation
Kyung Ohk AhnChul Woo Yang

Abstract

Nestin-expressing cells play a role in the repair process of injured tissues and organs. This study examined the nestin-expressing cells in interstitial fibrosis in experimental chronic cyclosporine A (CsA) nephropathy. Sprague Dawley rats were treated daily for 1 or 4 weeks with CsA (15 mg/kg) or vehicle (VH; olive oil, 1 mg/kg). Nestin mRNA expression was evaluated with reverse transcriptional-polymerase chain reaction, and nestin-expressing cells were detected immunohistochemically. Localization of nestin was performed with double labeling studies for vimentin, aquaporin 1, or calbindin D28K. Nestin mRNA expression was not different between VH- and CsA-treated rat kidneys. Nestin-expressing cells were rarely observed in the cortex in the VH group, but CsA-induced renal injury caused an increase in nestin-expressing cells in the cortex in a time-dependent manner. Nestin-expressing cells in the CsA group were localized to the area of interstitial fibrosis, and the number of nestin-expressing cells well correlated with the score of interstitial fibrosis (r=0.898). Nestin-expressing cells did not express vimentin, aquaporin 1, or calbindin D28K. CsA-induced renal injury recruits nestin-expressing cells to injured areas, and thes...Continue Reading

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Citations

Apr 5, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Michael SkwirbaVeronika Grau
Feb 11, 2012·Journal of Korean Medical Science·Jin Young KimChul Woo Yang
Jul 29, 2009·Nature Reviews. Nephrology·Can Li, Chul Woo Yang
Nov 28, 2019·Oxidative Medicine and Cellular Longevity·Kang LuoChul Woo Yang

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