Inflammation and activity augment brain-derived neurotrophic factor peripheral release

Neuroscience
L Y QiaoQ L Zhang

Abstract

Brain-derived neurotrophic factor (BDNF) release to nerve terminals in the central nervous system is crucial in synaptic transmission and neuronal plasticity. However, BDNF release peripherally from primary afferent neurons has not been investigated. In the present study, we show that BDNF is synthesized by primary afferent neurons located in the dorsal root ganglia (DRG) in rat, and releases to spinal nerve terminals in response to depolarization or visceral inflammation. In two-compartmented culture that separates DRG neuronal cell bodies and spinal nerve terminals, application of 50mM K(+) to either the nerve terminal or the cell body evokes BDNF release to the terminal compartment. Inflammatory stimulation of the visceral organ (e.g. the urinary bladder) also facilitates an increase in spontaneous BDNF release from the primary afferent neurons to the axonal terminals. In the inflamed viscera, we show that BDNF immunoreactivity is increased in nerve fibers that are immuno-positive to the neuronal marker PGP9.5. Both BDNF and pro-BDNF levels are increased, however, pro-BDNF immunoreactivity is not expressed in PGP9.5-positive nerve-fiber-like structures. Determination of receptor profiles in the inflamed bladder demonstrates ...Continue Reading

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Citations

Jun 18, 2019·Current Neuropharmacology·Ana CoelhoCélia Duarte Cruz
Aug 23, 2018·Memórias do Instituto Oswaldo Cruz·Henrique S CostaManoel Otavio C Rocha
Jul 12, 2021·Journal of Psychiatric Research·Mariane Nunes NotoRodrigo Affonseca Bressan

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