Inflammation-induced DNA methylation of DNA polymerase gamma alters the metabolic profile of colon tumors

Cancer & Metabolism
Ashley R MaiuriHeather M O'Hagan

Abstract

Inflammation, metabolism, and epigenetic modulation are highly interconnected processes that can be altered during tumorigenesis. However, because of the complexity of these interactions, direct cause and effect during tumorigenesis have been difficult to prove. Previously, using a murine model of inflammation-induced colon tumorigenesis, we determined that the promoter of the catalytic subunit of DNA polymerase gamma (Polg) is DNA hypermethylated and silenced in inflammation-induced tumors, but not in non-inflammation-induced (mock) tumors, suggesting that inflammation can induce silencing of Polg through promoting DNA methylation during tumorigenesis. Polg is the only mitochondrial DNA polymerase and mutations in Polg cause mitochondrial diseases in humans. Because of the role of mitochondria in metabolism, we hypothesized that silencing of Polg in inflammation-induced tumors would result in these tumors having altered metabolism in comparison to mock tumors. Inflammation-induced and mock colon tumors and colon epithelium from a mouse model of inflammation-induced colon tumorigenesis were assayed for alterations in Polg expression, mitochondria, and metabolism. Organoids derived from these tissues were used to study the direc...Continue Reading

References

May 13, 2005·Human Molecular Genetics·Nicole HanceAleksandra Trifunovic
Jul 25, 2009·Journal of Human Genetics·Keshav K SinghMarija Vujcic
Aug 4, 2011·Clinics·Suely Kazue Nagahashi Marie, Sueli Mieko Oba Shinjo
Mar 13, 2012·Seminars in Cell & Developmental Biology·Steven J Bensinger, Heather R Christofk
Jul 20, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tatianna C LarmanJ G Seidman
Sep 25, 2012·Nature Reviews. Cancer·Douglas C Wallace
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Lorenzo GalluzziGuido Kroemer
Apr 3, 2013·Cold Spring Harbor Perspectives in Biology·Jeffrey D StumpfWilliam C Copeland
May 1, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jana JeschkeNita Ahuja
May 15, 2013·Biophysical Journal·Salil P DesaiDaniel Irimia
May 29, 2013·Journal of Visualized Experiments : JoVE·Xiang Xue, Yatrik M Shah
Oct 16, 2013·Cancer Letters·Christelle JohnsonJason W Locasale
Dec 19, 2013·Frontiers in Oncology·Michelle L BolandKay F Macleod
Mar 19, 2014·Methods : a Companion to Methods in Enzymology·Jason M TennessenCarl S Thummel
Aug 12, 2014·Current Protocols in Mouse Biology·Maxime M MahéNoah F Shroyer
Oct 21, 2015·Frontiers in Cell and Developmental Biology·Elizabeth A Novak, Kevin P Mollen
Jan 16, 2016·Cell Metabolism·Natalya N Pavlova, Craig B Thompson
Jul 8, 2016·Science Advances·Ralph J DeBerardinis, Navdeep S Chandel
Oct 23, 2016·Trends in Endocrinology and Metabolism : TEM·Sukanya RaghuramanDavid Simar
Oct 25, 2016·Nature Reviews. Cancer·Adam KinnairdEvangelos D Michelakis

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Citations

Mar 28, 2019·Gut Microbes·Dakota N Jackson, Arianne L Theiss
Jun 27, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Ashley R MaiuriHeather M O'Hagan
Feb 25, 2021·Cancer Management and Research·Mengzhu LvShu Guo
Dec 24, 2020·Brain Pathology·Carola Hedberg-OldforsAnders Oldfors

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Methods Mentioned

BETA
scraping
PCR
Assay
transmission electron microscopy

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