PMID: 7539915Jun 6, 1995Paper

Inflammation-induced recombinant protein expression in vivo using promoters from acute-phase protein genes

Proceedings of the National Academy of Sciences of the United States of America
A W VarleyR S Munford

Abstract

We report that promoters for two murine acute-phase protein (APP) genes, complement factor 3 (C3) and serum amyloid A3 (SAA3), can increase recombinant protein expression in response to inflammatory stimuli in vivo. To deliver APP promoter-luciferase reporter gene constructs to the liver, where most endogenous APP synthesis occurs, we introduced them into a nonreplicating adenovirus vector and injected the purified viruses intravenously into mice. When compared with the low levels of basal luciferase expression observed prior to inflammatory challenge, markedly increased expression from the C3 promoter was detected in liver in response to both lipopolysaccharide (LPS) and turpentine, and lower-level inducible expression was also found in lung. In contrast, expression from the SAA3 promoter was found only in liver and was much more responsive to LPS than to turpentine. After LPS challenge, hepatic luciferase expression increased rapidly and in proportion to the LPS dose. Use of cytokine-inducible promoters in gene transfer vectors may make it possible to produce antiinflammatory proteins in vivo in direct relationship to the intensity and duration of an individual's inflammatory response. By providing endogenously controlled pro...Continue Reading

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Citations

Oct 30, 1998·Molecular Medicine Today·A W Varley, R S Munford
Feb 9, 2010·Cancer Gene Therapy·R T HoggR D Gerard
Aug 20, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jeroen GeurtsFons A J van de Loo
Oct 23, 1997·Nature Biotechnology·A W VarleyR S Munford
Dec 9, 1998·American Journal of Respiratory Cell and Molecular Biology·K NarumiR G Crystal
May 8, 2002·The Journal of Clinical Investigation·A V MiagkovS S Makarov
Sep 24, 2004·Arthritis Research & Therapy·Fons A J van de LooWim B van den Berg
Sep 3, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Chia-Hung LinShung-Te Kao
Sep 16, 2006·Current Rheumatology Reports·Fons A J van de LooWim B van den Berg
Feb 6, 2007·Journal of Ethnopharmacology·Chia-Chou YehShung-Te Kao
Nov 28, 2000·Baillière's Best Practice & Research. Clinical Rheumatology·W B van den Berg
May 15, 2001·Toxicology and Applied Pharmacology·J CowanR S Munford
Jun 9, 2016·The Journal of Gene Medicine·Simone GabnerMonika Egerbacher
Jul 5, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Nuno Araújo-GomesIsabel Goñi
Jul 14, 2004·Medicinal Research Reviews·Hai-sheng ZhouChih-chuan Liang
Feb 12, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·I J KulloR S Schwartz
Jul 9, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshihiro FukuokaLawrence B Schwartz
Jan 31, 1998·Cardiovascular Research·R D Gerard, D Collen

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