Inflammation-related differences in mucosa-associated microbiota and intestinal barrier function in colonic Crohn's disease

American Journal of Physiology. Gastrointestinal and Liver Physiology
Josie LibertucciDavid Armstrong

Abstract

Crohn's disease (CD), characterized by discontinuous intestinal injury and inflammation, has been associated with changes in luminal microbial composition and impaired barrier function. The relationships between visual features of intestinal injury, permeability, and the mucosa-associated microbiota are unclear. Individuals undergoing routine colonoscopy (controls) and patients with CD were evaluated by clinical parameters and confocal laser scanning endomicroscopic colonoscopy (CLE). Patients with CD were categorized as either CD with no injury (CD-NI) or CD with injury (CD-I). Colonic biopsies were taken from adjacent matched sites in all individuals, and CLE images from these sites were analyzed for vascular permeability. Microbial composition was evaluated by 16S rRNA gene sequencing of the V3 region, and the mycome was identified through internal transcribed spacer 2 sequencing. Subgroup analyses were performed for histology, paracellular permeability (Ussing chamber), and encroachment of bacteria (fluorescent in situ hybridization). CD-I patients showed an altered microbial community compared with both controls and CD-NI patients, with enrichment in Escherichia and a decrease in Firmicutes, including Lachnospira, Faecalib...Continue Reading

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Methods Mentioned

BETA
biopsy
biopsies
sedation
ELISA
electrophoresis
fluorescence microscopy

Software Mentioned

Cutadapt
IMAGE J
CLE
fastq
PANDAseq

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