Inflammation-related microRNA expression level in the bovine milk is affected by mastitis

PloS One
Yu-Chang LaiNaoki Miura

Abstract

MicroRNA (miRNA) in tissue and liquid samples have been shown to be associated with many diseases including inflammation. We aimed to identify inflammation-related miRNA expression level in the bovine mastitis milk. Expression level of inflammation-related miRNA in milk from mastitis-affected and normal cows was analyzed using qPCR. We found that expression level of miR-21, miR-146a, miR-155, miR-222, and miR-383 was significantly upregulated in California mastitis test positive (CMT+) milk. We further analyzed these miRNA using a chip-based QuantStudio Digital PCR System. The digital PCR results correlated with those of qPCR, demonstrating upregulation of miR-21, miR-146a, miR-155, miR-222, and miR-383 in CMT+ milk. In conclusion, we identified miRNA that are upregulated in CMT+ milk. These miRNA exhibited sensitivity and specificity greater than 80% for differentiating between CMT+ milk and normal milk. Our findings suggest that inflammation-related miRNA expression level in the bovine milk was affected by mastitis, and miRNA in milk have potential for use as biomarkers of bovine mastitis.

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Citations

Mar 26, 2019·Annual Review of Food Science and Technology·Grace J AhernCatherine Stanton
Oct 31, 2019·The FEBS Journal·Yu-Chang LaiNaoki Miura
Apr 29, 2020·Journal of Veterinary Internal Medicine·Eric J FishRichard Curt Bird
Apr 14, 2018·Frontiers in Cellular and Infection Microbiology·Jizong LiJieyuan Jiang
Mar 4, 2020·Mikrochimica Acta·Adrián Sánchez-VisedoMaría Teresa Fernández-Argüelles
Jan 5, 2021·Frontiers in Veterinary Science·Silvia MirettiMario Baratta
Nov 10, 2020·Research in Veterinary Science·Yu-Chang LaiNaoki Miura
May 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Abderrahim Benmoussa, Patrick Provost
Apr 4, 2021·International Journal of Molecular Sciences·Duy N DoEveline M Ibeagha-Awemu
Oct 13, 2021·Journal, Genetic Engineering & Biotechnology·Farmanullah FarmanullahZhang Shujun

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Methods Mentioned

BETA
PCR
Assay
chips
biopsy

Software Mentioned

QuantStudio 3D
QuantStudio
QuantStudio 3D Digital PCR
GraphPad Prism
QuantStudio 3D Analysis Suite
GraphPad

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