Inflammatory and fibrotic proteins proteomically identified as key protein constituents in urine and stone matrix of patients with kidney calculi

Clinica Chimica Acta; International Journal of Clinical Chemistry
Chanchai BoonlaKerstin Krieglstein

Abstract

To uncover whether urinary proteins are incorporated into stones, the proteomic profiles of kidney stones and urine collected from the same patients have to be explored. We employed 1D-PAGE and nanoHPLC-ESI-MS/MS to analyze the proteomes of kidney stone matrix (n=16), nephrolithiatic urine (n=14) and healthy urine (n=3). We identified 62, 66 and 22 proteins in stone matrix, nephrolithiatic urine and healthy urine, respectively. Inflammation- and fibrosis-associated proteins were frequently detected in the stone matrix and nephrolithiatic urine. Eighteen proteins were exclusively found in the stone matrix and nephrolithiatic urine, considered as candidate biomarkers for kidney stone formation. S100A8 and fibronectin, representatives of inflammation and fibrosis, respectively, were up-regulated in nephrolithiasis renal tissues. S100A8 was strongly expressed in infiltrated leukocytes. Fibronectin was over-expressed in renal tubular cells. S100A8 and fibronectin were immunologically confirmed to exist in nephrolithiatic urine and stone matrix, but in healthy urine they were undetectable. Conclusion, both kidney stones and urine obtained from the same patients greatly contained inflammatory and fibrotic proteins. S100A8 and fibronec...Continue Reading

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Citations

Jul 16, 2014·Urolithiasis·Wipawee KittikowitPiyaratana Tosukhowong
Apr 19, 2015·Journal of Translational Medicine·Yanbao YuRembert Pieper
Feb 1, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Supaporn KhamchunVisith Thongboonkerd
Mar 1, 2016·Proteome Science·Frank A WitzmannJames C Williams
Dec 14, 2019·Current Opinion in Urology·Wilson Sui, Ryan S Hsi
Jun 9, 2020·BioMed Research International·Yanan CaoZhitao Dong
Dec 4, 2016·Urolithiasis·Jeffrey D RimerJeffrey A Wesson
Nov 25, 2018·Nature Reviews. Urology·Erika J Espinosa-OrtizRobin Gerlach
Jan 31, 2021·Nature Reviews. Nephrology·Saeed R KhanPaul R Dominguez-Gutierrez

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