Inflammatory cytokines (IL-1alpha, TNF-alpha) and LPS modulate the Ca2+ signaling pathway in osteoblasts

The American Journal of Physiology
V K TamJ Green


Locally derived growth factors and cytokines in bone play a crucial role in the regulation of bone remodeling, i.e., bone formation and bone resorption processes. We studied the effect of interleukin (IL)-1alpha, tumor necrosis factor (TNF)-alpha, and Escherichia coli lipopolysaccharide (LPS) on the hormone-activated Ca2+ message system in the osteoblastic cell line UMR-106 and in osteoblastic cultures derived from neonatal rat calvariae. In both cell preparations, IL-1alpha, TNF-alpha, and LPS did not alter basal intracellular Ca2+ concentration ([Ca2+]i) but attenuated Ca2+ transients evoked by parathyroid hormone (PTH) and PGE2 in a dose (1-100 ng/ml)- and time (8-24 h)-dependent fashion. The cytokines modulated hormonally induced Ca2+ influx (estimated by using Mn2+ as a surrogate for Ca2+) as well as Ca2+ mobilization from intracellular stores. The latter was linked to suppressed production of hormonally induced inositol 1,4,5-trisphosphate. The effect of cytokines on [Ca2+]i was abolished by the tyrosine kinase inhibitor herbimycin A (50 ng/ml). The cytokine's effect was, however, independent of nitric oxide (NO) production, since NO donors (sodium nitroprusside) as well as permeable cGMP analogs augment, rather than atte...Continue Reading


Dec 1, 1976·The Journal of Clinical Investigation·J L IveyA H Tashjian
Sep 4, 1992·Cell·C J Lowenstein, S H Snyder
Jan 1, 1991·Methods in Enzymology·Y Uehara, H Fukazawa
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I MacIntyreJ R Vane
Mar 1, 1991·Endocrinology·M Mengozzi, P Ghezzi
Feb 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T AkatsuT Suda
Apr 1, 1990·The Biochemical Journal·A W SimpsonC C Ashley
Feb 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J PfeilschifterG D Roodman
Feb 1, 1988·The Journal of Clinical Investigation·E CanalisM Centrella
Feb 1, 1986·Endocrinology·P M McSheehy, T J Chambers
Jul 1, 1986·The Journal of Experimental Medicine·B M ThomsonT J Chambers
Mar 1, 1983·The Journal of Cell Biology·B Ecarot-CharrierG Pereira
Jun 15, 1994·Biochemical and Biophysical Research Communications·P D Damoulis, P V Hauschka
Oct 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C D HanevoldS C Jordan
Feb 29, 1996·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J SaklatvalaF Guesdon
Sep 18, 1997·The Journal of Clinical Investigation·Y Abu-AmerS L Teitelbaum

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