Inflammatory Drivers of Cardiovascular Disease: Molecular Characterization of Senescent Coronary Vascular Smooth Muscle Cells

Frontiers in Physiology
Stevan D StojanovićThomas Thum

Abstract

The senescence of vascular smooth muscle cells (VSMCs) has been implicated as a causal pro-inflammatory mechanism for cardiovascular disease development and progression of atherosclerosis, the instigator of ischemic heart disease. Contemporary limitations related to studying this cellular population and senescence-related therapeutics are caused by a lack of specific markers enabling their detection. Therefore, we aimed to profile a phenotypical and molecular signature of senescent VSMCs to allow reliable identification. To achieve this goal, we have compared non-senescent and senescent VSMCs from two in vitro models of senescence, replicative senescence (RS) and DNA-damage induced senescence (DS), by analyzing the expressions of established senescence markers: cell cycle inhibitors- p16 INK4a, p14 ARF, p21 and p53; pro-inflammatory factors-Interleukin 1β (IL-1β), IL-6 and high mobility group box-1 (HMGB-1); contractile proteins-smooth muscle heavy chain- (MYH11), smoothelin and transgelin (TAGLN), as well as structural features (nuclear morphology and LMNB1 (Lamin B1) expression). The different senescence-inducing modalities resulted in a lack of the proliferative activity. Nucleomegaly was seen in senescent VSMC as compared t...Continue Reading

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Citations

Jun 13, 2020·International Journal of Molecular Sciences·Stevan D StojanovićMeik Kunz
Dec 31, 2020·BioMed Research International·Pan HuangMin Xu
Apr 4, 2021·International Journal of Molecular Sciences·Yu-Jih SuShao-Wen Weng
Jun 19, 2021·Cardiovascular Research·Abel Martin GarridoMartin Bennett
Aug 13, 2021·Chemical Research in Toxicology·Xijun ZhangHaohui Zhu
Aug 14, 2021·Frontiers in Cell and Developmental Biology·Tianbin ChenLi Zhang

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Methods Mentioned

BETA
electrophoresis
immunoprecipitation
Profiler

Software Mentioned

Image J
R
MaxQuant
ggplot2
Perseus
Andromeda
: Profiler
Graph Pad Prism

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