PMID: 15223808Jun 30, 2004Paper

Inflammatory mediators in autoimmune lacrimal gland disease in MRL/Mpj mice

Investigative Ophthalmology & Visual Science
D A JabsJ A Whittum-Hudson

Abstract

MRL/MpJ-fas(+)/fas(+) (MRL/+) and MRL/MpJ-fas(lpr)/fas(lpr) (MRL/lpr) mice are congenic substrains of mice that have spontaneously developing lacrimal and salivary gland inflammation and are models for the human disorder Sjögren's syndrome. Nitric oxide (NO) and tumor necrosis factor (TNF)-alpha are proinflammatory and potential mediators of tissue damage. The presence of the inducible form of nitric oxide synthase (iNOS), which catalyzes the production of NO, and the presence TNF-alpha in the lacrimal glands of MRL/MpJ mice were assessed. Lacrimal glands from MRL/+ and MRL/lpr mice, at ages 1 through 9 months, were evaluated by real-time RT-PCR for iNOS and TNF-alpha mRNA and by immunohistochemistry for the presence of iNOS and of TNF-alpha. Age-matched BALB/c lacrimal glands were used as the control. By quantitative real-time PCR (qPCR), mRNA for iNOS was detected in the lacrimal glands in significantly greater amounts in both MRL/+ (median, normalized to 18S rRNA, 2.90; P < 0.0003) and MRL/lpr mice (median 6.84, P < 0.001) than in BALB/c mice (median 0.34). By qPCR, mRNA for TNF-alpha in the lacrimal glands was detected in significantly greater amounts in aged MRL/+ mice than in BALB/c mice (median, normalized to actin, 221....Continue Reading

Citations

Mar 22, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sabrina ViauCorinne Joffre
Mar 14, 2013·Investigative Ophthalmology & Visual Science·Atsuko FujiiMitsuyoshi Azuma
Sep 17, 2011·Journal of the American Society of Nephrology : JASN·Hiroyuki MatsudaJohanne Tremblay
Feb 27, 2008·The Veterinary Clinics of North America. Small Animal Practice·David L Williams
Mar 25, 2010·Journal of Cellular Physiology·Sze-Ying NgSuk-Ying Tsang
Jun 11, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroko AdachiKei Tashiro

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