Inflammatory microenvironment and tumor necrosis factor alpha as modulators of periostin and CCN2 expression in human non-healing skin wounds and dermal fibroblasts

Matrix Biology : Journal of the International Society for Matrix Biology
Christopher ElliottDouglas W Hamilton

Abstract

Non-healing skin wounds remain a significant clinical burden, and in recent years, the regulatory role of matricellular proteins in skin healing has received significant attention. Periostin and CCN2 are both upregulated at day 3 post-wounding in murine skin, where they regulate aspects of the proliferative phase of repair including mesenchymal cell infiltration and myofibroblast differentiation. In this study, we examined 1) the wound phenotype and expression patterns of periostin and CCN2 in non-healing skin wounds in humans and 2) the regulation of their expression in wound fibroblasts by tumor necrosis factor α (TNFα) and transforming growth factor-β1 (TGF-β1). Chronic skin wounds had a pro-inflammatory phenotype, characterized by macrophage infiltration, TNFα immunoreactivity, and neutrophil infiltration. Periostin, but not CCN2, was significantly suppressed in non-healing wound edge tissue at the mRNA and protein level compared with non-involved skin. In vitro, human wound edge fibroblasts populations were still able to proliferate and contract collagen gels. Compared to cells from non-involved skin, periostin and α-SMA mRNA levels increased significantly in the presence of TGF-β1 in wound cells and were significantly dec...Continue Reading

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Citations

Feb 2, 2016·The Journal of Investigative Dermatology·John T WalkerDouglas W Hamilton
May 29, 2016·Cell and Tissue Research·John T WalkerDouglas W Hamilton
Jan 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Massimiliano GasparriniMaurizio Battino
May 5, 2017·Cutaneous and Ocular Toxicology·Sibel Berksoy HaytaMelih Akyol
Jan 27, 2018·Journal of Cell Communication and Signaling·Karrington McLeodDouglas W Hamilton
Apr 9, 2020·American Journal of Physiology. Cell Physiology·Georgia NikoloudakiDouglas W Hamilton
Sep 3, 2020·Vascular Medicine·Jiri Kanta, Anna Zavadakova
Dec 24, 2018·Tissue Engineering. Part a·Christopher G ElliottDouglas W Hamilton
Dec 6, 2019·Journal of Traditional and Complementary Medicine·Swagata MazumdarParimal Karmakar
Oct 29, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Douglas W HamiltonLauren E Flynn

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