Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease

BMC Veterinary Research
Manuel R SchmidliSimone Forterre

Abstract

Despite the importance of inflammation during the pathogenesis of cranial cruciate ligament disease (CCLD) in dogs and despite the latest knowledge suggesting a significant role of adipose tissue in osteoarthritis, the infrapatellar fat pad (IFP) was up to now mostly disregarded in veterinary investigations. In the present study, the inflammatory activity of the IFP, the main adipose structure within the stifle joint, was thoroughly investigated to evaluate its potential impact in the pathogenesis of this common disease of our canine companions. Samples of IFP, subcutaneous adipose tissue (ScAT) of the thigh and synovial fluid in both diseased (n = 36) and healthy control (n = 23) dogs were tested for their immune cell composition but also for interleukins (IL-1β, IL-6, IL-8, IL-10), degradative enzymes (MMP-1, MMP-3, MMP-13, TIMP-2, iNOS) and adipokines (leptin and adiponectin). Characterization of the immune cell composition was ascertained by fluorescence activated cell sorting. Gene expression and protein release of the inflammatory markers was determined by real RT-qPCR and ELISA. IFPs of dogs with CCLD had a significantly increased immune cell count with T cells (CD3) as the most abundant immune cells. T cells and macroph...Continue Reading

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Citations

Mar 20, 2020·Mediators of Inflammation·Yoke Yue Chow, Kok-Yong Chin
Oct 9, 2020·Scientific Reports·Yuyan WangHeidi L Reesink
Feb 12, 2021·Journal of Feline Medicine and Surgery·Evangelia ManiakiEmily J Blackwell
Jan 12, 2020·Osteoarthritis and Cartilage·C-L WuF Guilak

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Methods Mentioned

BETA
percutaneous arthrocentesis
flow
ELISA
PCR
flow cytometry
FACS

Software Mentioned

MedCalc
ScAT
7300 System Detection
Diva

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