Inflammatory response and biomechanical properties of coaxial scaffolds for engineered skin in vitro and post-grafting

Acta Biomaterialia
Britani N BlackstoneHeather M Powell

Abstract

Engineered skin (ES) offers many advantages over split-thickness skin autografts for the treatment of burn wounds. However, ES, both in vitro and after grafting, is often significantly weaker, less elastic and more compliant than normal human skin. Biomechanical properties of ES can be tuned in vitro using electrospun co-axial (CoA) scaffolds. To explore the potential for coaxial scaffold-based ES use in vivo, two CoA scaffolds were fabricated with bioactive gelatin shells and biodegradable synthetic cores of polylactic acid (PLA) and polycaprolactone (PCL), and compared with gelatin monofilament scaffolds. Fibroblast and macrophage production of inflammatory cytokines interleukin 6 (IL-6) and transforming growth factor β-1 was significantly higher when cultured on PLA and PCL monofilament scaffolds compared to gelatin monofilament scaffolds. The core-shell fiber configuration significantly reduced production of pro-inflammatory cytokines to levels similar to those of gelatin monofilament scaffolds. In vitro, ES mechanical properties were significantly enhanced using CoA scaffolds; however, after grafting CoA- and gelatin-based ES to full-thickness excisional wounds on athymic mice, the in vitro mechanical advantage of CoA graf...Continue Reading

Citations

May 21, 2019·Journal of Biomaterials Science. Polymer Edition·Thalles Canton TrevisolAna Paula Serafini Immich
Oct 13, 2020·Frontiers in Bioengineering and Biotechnology·Naresh KumarMahmood Khan
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Divya SridharanMahmood Khan

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