Inflammatory responses in aggregating rat brain cell cultures subjected to different demyelinating conditions

Brain Research
Antoinette DefauxFlorianne Monnet-Tschudi

Abstract

To study inflammatory reactions occurring in relation to demyelination, aggregating rat brain cell cultures were subjected to three different demyelinating insults, i.e., (i) lysophosphatidylcholine (LPC), (ii) interferon-gamma combined with lipopolysaccharide (IFN-gamma+LPS), and (iii) anti-MOG antibodies plus complement (alpha-MOG+C). Demyelination was assessed by measuring the expression of myelin basic protein (MBP) and myelin oligodendrocyte glycoprotein (MOG), and the activity of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP). The accompanying inflammatory reactions were examined by the quantification of microglia-specific staining, by immunostaining for glial fibrillary acidic protein (GFAP), and by measuring the mRNA expression of a panel of inflammation-related genes. It was found that all three demyelinating insults decreased the expression of MBP and MOG, and induced microglial reactivity. LPC and alpha-MOG+C, but not IFN-gamma+LPS, decreased CNP activity; they also caused the appearance of macrophagic microglia, and increased GFAP staining indicating astrogliosis. LPC affected also the integrity of neurons and astrocytes. LPC and IFN-gamma+LPS upregulated the expression of the inflammation-related genes IL-6, TNF...Continue Reading

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Citations

Jul 7, 2011·Journal of Neuroinflammation·Samuel J JacksonDavid Baker
Mar 19, 2013·Theoretical Biology & Medical Modelling·Maria RennerAnnette Kopp-Schneider
Nov 8, 2011·Current Protocols in Toxicology·Florianne Monnet-TschudiMarie-Gabrielle Zurich
Feb 13, 2020·Journal of Clinical Medicine·Marcelo Duarte AzevedoLiliane Tenenbaum

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