Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration

Journal of Neuroimmunology
Lisa McCluskeyStuart M Allan

Abstract

Direct intra-cerebral administration of substances into the brain parenchyma is a common technique used by researchers in neuroscience. However, inflammatory responses to the needle may confound the results obtained following injection of these substances. In this paper we show that the use of a glass micro-needle for intra-cerebral injection reduces mechanical injury, blood-brain barrier breakdown and neutrophil recruitment in response to the injection of vehicle or interleukin-1, compared to using a 26-gauge Hamilton syringe. Therefore, the use of a glass micro-needle to inject substances intra-cerebrally appears to cause minimal injection artefact and should be the method of choice.

References

Sep 1, 1992·Neurochemical Research·W T NortonC F Brosnan
Oct 1, 1995·Current Opinion in Neurobiology·V H PerryM K Matyszak
Apr 8, 1999·Experimental Neurology·J N TurnerH Craighead
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S M AllanN J Rothwell
Feb 4, 2003·Nature Reviews. Neuroscience·V Hugh PerryColm Cunningham
Feb 13, 2003·Journal of Neuroscience Methods·Bassam N Hamam, Timothy E Kennedy
Jan 24, 2007·Neuropathology and Applied Neurobiology·S J CampbellD C Anthony

❮ Previous
Next ❯

Citations

Jun 6, 2013·Translational Neurodegeneration·Johannes Pjm de MunterErik Ch Wolters
May 6, 2014·Brain Research·Patrick LoseyDaniel C Anthony
Jul 2, 2011·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Grant McAuleyWolff Kirsch
Aug 25, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Matthew A KirkmanAdrian R Parry-Jones
Feb 1, 2018·Stroke; a Journal of Cerebral Circulation·Megan A EvansBrad R S Broughton
Jun 5, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charlotte AllenStuart M Allan
Jul 6, 2019·British Journal of Pharmacology·Camilo RojasBarbara Slusher
Jul 10, 2019·Contrast Media & Molecular Imaging·Min ZhangFabien Chauveau
Aug 12, 2017·Journal of Extracellular Vesicles·Guoku HuShilpa Buch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier & Cytokines

Some cytokines are able to cross the blood brain barrier through transport systems and enter the cerebrospinal fluid and interstitial fluid spaces. Here is the latest research on cytokines crossing the blood brain barrier and how this can affect tissues within the CNS.