Inflammatory responses involving tumor necrosis factor receptor-associated factor 6 contribute to in-stent lesion formation in a stent implantation model of rabbit carotid artery

Journal of Vascular Surgery
Takuya MiyaharaHirokazu Nagawa

Abstract

Inflammatory responses are considered to represent a unique property after stent implantation, and we previously demonstrated that inflammatory signaling involving tumor necrosis factor receptor-associated factor 6 (TRAF6) contributes to neointimal formation in a balloon injury model of rabbit carotid artery. The purpose of this study was to examine the role of TRAF6 in in-stent lesion formation after stent implantation in the rabbit carotid artery. Rabbit carotid arteries were injured with a 2F Fogarty catheter, and 28 days later, the same arteries were implanted with a 3-mm-diameter Palmaz-Schatz stent. A dominant negative (DN) form of TRAF6 (pME-FLAG-T6deltaRZ5) was then transferred using a plasmid-based electroporation method. Its effects were evaluated compared with the findings in arteries treated with control plasmid (pME-FLAG). Immunostaining with anti-FLAG tag antibody showed that an expression plasmid vector containing the DN-TRAF6 sequence was successfully transferred to the arterial intima and media. Morphometric analyses revealed that the increase of intimal area in in-stent lesions was significantly inhibited by DN-TRAF6 14 days after stent implantation (DN-TRAF6 group, 3.01 +/- 0.25 x 10(5) microm2 vs control gro...Continue Reading

References

Feb 1, 1987·The Journal of Clinical Investigation·C F Nathan
Sep 1, 1995·Journal of the American College of Cardiology·G R DussaillantM B Leon
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z S GalisP Libby
Oct 3, 1996·Nature·Z CaoD V Goeddel
Nov 15, 1996·The Journal of Clinical Investigation·M PoonA R Marks
Jun 9, 1998·Heart·A H Gershlick, J Baron
Dec 31, 2002·Journal of the American College of Cardiology·Ick-Mo ChungThomas N Wight
Aug 11, 2004·Circulation·Andrew FarbRenu Virmani

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Citations

Sep 16, 2006·Human Gene Therapy·Loree C Heller, Richard Heller
May 6, 2016·Basic Research in Cardiology·Matthijs F JansenEsther Lutgens
Nov 28, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Muhammad AbdullahMonte S Willis

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