Inflammatory stress induces lipid accumulation in multi-organs of db/db mice

Acta Biochimica Et Biophysica Sinica
Kun Ling MaBi Cheng Liu

Abstract

Dyslipidemia and chronic inflammation play crucial roles in the progression of diabetes. This study aimed to investigate the effects of inflammatory stress on lipid accumulation in multi-organs in diabetes. Eight-week-old male db/db mice were randomly assigned to inflamed group with alternating day subcutaneous injection of 10% casein or control group with daily injection of distilled water. The lipid profile and plasma levels of inflammatory cytokines were determined using a clinical biochemical assay and enzyme-linked immunosorbent assay. The effects of inflammation on lipid accumulation in target organs were evaluated by hematoxylin-eosin staining, Oil Red O staining, Filipin staining, and a quantitative intracellular cholesterol assay. The protein expressions of low-density lipoprotein receptor (LDLr), sterol regulatory element binding protein-2 (SREBP-2), and SREBP-cleavage-activating protein (SCAP) in tissues were assessed by immunohistochemical staining and western blotting. Results showed that the serum levels of inflammatory cytokines were significantly elevated in casein-injected mice, suggesting that an inflamed diabetic model was established. Furthermore, the protein expressions of inflammatory cytokines in aortas, ...Continue Reading

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Citations

Feb 23, 2019·Journal of Lipid Research·Hongwu WangQin Ning
Jul 8, 2016·American Journal of Physiology. Renal Physiology·Maria MustafaJoan C Krepinsky
May 1, 2019·Journal of Lipid Research·Hongwu WangQin Ning

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