Apr 16, 2016

Influence of 6 different intestinal bacteria on Beltsville Small White turkey semen

Poultry Science
M D TriplettA S Kiess


The turkey industry relies totally on artificial insemination to continue and improve production. If something compromises the insemination process, such as contaminated semen, it becomes a detrimental loss to the industry. Bacteria have been found in broiler breeder males to reduce sperm motility. The Sperm Quality Index (SQI) is a quick method to determine avian sperm motility using the sperm quality analyzer (SQA). Therefore, the objective of this study was to determine if bacteria have an effect on turkey sperm motility using the SQA. For the experiment, one mL of pooled neat semen was collected from Beltsville Small White Turkey toms. Six intestinal bacteria, Bifidobacterium animalis, Campylobacter jejuni, Clostridium bifermentans, Escherichia coli, Lactobacillus acidophilus, and Salmonella enterica were grown overnight. For each bacterium, 4 treatments were made that consisted of exposing pooled semen to either saline, sterile broth, an overnight culture of each individual bacterium, or a centrifuged pellet of each bacterium re-suspended in saline. The experiment was repeated 3 times. Once the semen was exposed to the respective treatment, a portion was pulled into a capillary tube and placed into the SQA to obtain the SQ...Continue Reading

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Mentioned in this Paper

Seminal Fluid
Bifidobacterium animalis
Alkalescens-Dispar Group
Pellet Formation
Cell Motility
Salmonella enterica
Sperm Cell

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