Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase.

Applied and Environmental Microbiology
Kimiya MizutaniKazuo Sakka

Abstract

In general, cellulases and hemicellulases are modular enzymes in which the catalytic domain is appended to one or more noncatalytic carbohydrate binding modules (CBMs). CBMs, by concentrating the parental enzyme at their target polysaccharide, increase the capacity of the catalytic module to bind the substrate, leading to a potentiation in catalysis. Clostridium thermocellum hypothetical protein Cthe_0821, defined here as C. thermocellum Man5A, is a modular protein comprising an N-terminal signal peptide, a family 5 glycoside hydrolase (GH5) catalytic module, a family 32 CBM (CBM32), and a C-terminal type I dockerin module. Recent proteomic studies revealed that Cthe_0821 is one of the major cellulosomal enzymes when C. thermocellum is cultured on cellulose. Here we show that the GH5 catalytic module of Cthe_0821 displays endomannanase activity. C. thermocellum Man5A hydrolyzes soluble konjac glucomannan, soluble carob galactomannan, and insoluble ivory nut mannan but does not attack the highly galactosylated mannan from guar gum, suggesting that the enzyme prefers unsubstituted β-1,4-mannoside linkages. The CBM32 of C. thermocellum Man5A displays a preference for the nonreducing ends of mannooligosaccharides, although the prot...Continue Reading

References

Nov 1, 1992·Applied and Environmental Microbiology·G MaglioneD B Wilson
Jul 11, 2002·Journal of Agricultural and Food Chemistry·Piet J H DaasHarmen H J De Jongh
Oct 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Simon L NewsteadGarry Taylor
Sep 23, 2006·The Journal of Biological Chemistry·Elizabeth Ficko-Blean, Alisdair B Boraston
Jul 24, 2007·Journal of Bacteriology·Nicholas D Gold, Vincent J J Martin
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Dec 25, 2008·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Edward A BayerHarry J Flint
May 16, 2009·Biochemistry·Louise E TailfordHarry J Gilbert
Apr 9, 2010·Annual Review of Biochemistry·Carlos M G A Fontes, Harry J Gilbert
Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Makiko SakkaKazuo Sakka

❮ Previous
Next ❯

Citations

Jun 14, 2002·Archives of Biochemistry and Biophysics·P I Castagnet, N M Giusto
Oct 27, 2015·Bioprocess and Biosystems Engineering·Burcu Gündüz Ergün, Pınar Çalık
Oct 15, 2013·FEMS Microbiology Reviews·Sara E Blumer-SchuetteRobert M Kelly
May 20, 2016·Applied Microbiology and Biotechnology·Constantinos KatsimpourasEvangelos Topakas
Aug 30, 2013·The Journal of Biological Chemistry·Shoko ShinyaTamo Fukamizo
Oct 25, 2014·The Journal of Biological Chemistry·Jonathan R ChekanSatish K Nair
Apr 24, 2018·Applied Microbiology and Biotechnology·Johan MorrillHenrik Stålbrand
Oct 10, 2020·Critical Reviews in Biotechnology·Gaurav Singh Kaira, Mukesh Kapoor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.