Influence of acinar tissue contamination on encapsulated pancreatic islets: morphological and functional studies

Transplantation
L KesslerM Pinget

Abstract

The present study concerns the influence of acinar contamination on pancreatic islets encapsulated in an artificial membrane (AN 69). Pure, handpicked pancreatic islets were contaminated by the addition of acinar tissue (ratio, 1:1). The morphological aspect and insulin release of both pure (n=12) and contaminated (n=12) encapsulated islets were assessed after 10 days of culture or implantation in the peritoneal cavity of rats. After implantation, the encapsulated islets, irrespective of their purity, were totally altered, whereas the morphological aspect of the cultivated islets remained intact only in the absence of acinar tissue contamination. This contamination induced a significant decrease in the stimulation index of insulin release. The stimulation index decreased by 42% for fresh islets and by 52% and 34% for cultivated and implanted islets, respectively, without modification in their basal release of insulin. The acinar tissue proved detrimental to the encapsulated implanted and cultivated islets.

Citations

Sep 20, 2012·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Leonardo RicottiArianna Menciassi
Dec 4, 2009·Biological Procedures Online·Jeffrey D CarterCraig S Nunemaker
Jun 3, 2004·Biomaterials·Meirigeng QiKazutomo Inoue
Mar 29, 2001·Differentiation; Research in Biological Diversity·B M SchmiedP M Pour
Jul 28, 2011·Pancreas·Orison O WoolcottRobert H Chow
May 29, 2000·Pancreas·B M SchmiedP M Pour
Nov 29, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Youakim SalibaNassim Farès
Mar 2, 2021·Biological Procedures Online·Kathryn L CorbinCraig S Nunemaker
Jun 15, 2005·Journal of Proteome Research·Meftun AhmedPeter Bergsten

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