Influence of Acyl Chain Saturation on the Membrane-Binding Activity of a Short Antimicrobial Peptide

ACS Omega
Daniela CiumacJian R Lu

Abstract

Different bacterial types and their living environments can lead to different saturations in the chains of their membrane lipids. Such structural differences may influence the efficacy of antibiotics that target bacterial membranes. In this work, the effects of acyl chain saturation on the binding of an antimicrobial peptide G4 have been examined as a function of the packing density of lipid monolayers by combining external reflection Fourier transform infrared (ER-FTIR) spectroscopy and neutron reflection (NR) measurements. Langmuir monolayers were formed from 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG), respectively, with the initial surface pressures controlled at 8 and 28 mN/m. A reduction in the order of the acyl chains associated with the increase in the layer thickness upon G4 binding was revealed from ER-FTIR spectroscopy, with peptide binding reaching equilibration faster in POPG than in DPPG monolayers. Whereas the dynamic DPPG-binding process displayed a steady increase in the amide I band area, the POPG-binding process showed little change in the amide area after the initial period. The peptide amide I area from ER-FTIR spectros...Continue Reading

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Citations

Feb 26, 2019·Future Medicinal Chemistry·Catarina Dias, Amélia P Rauter
Nov 16, 2018·Journal of Colloid and Interface Science·Daniela CiumacJian Ren Lu
Feb 12, 2020·Advances in Colloid and Interface Science·Luke A CliftonMarité Cárdenas
May 8, 2018·Journal of Colloid and Interface Science·Petru NigaC Magnus Johnson
Dec 2, 2020·ACS Applied Materials & Interfaces·Haoning GongJian Ren Lu
Sep 11, 2020·ACS Applied Materials & Interfaces·Haoning GongJian Ren Lu
Aug 27, 2019·ACS Applied Materials & Interfaces·Haoning GongJian Ren Lu
Aug 14, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Cuixia ChenHai Xu

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Methods Mentioned

BETA
fluorescence microscopy
circular dichroism

Software Mentioned

OMNIC
Motofit

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