Influence of Adhesion Force on icaA and cidA Gene Expression and Production of Matrix Components in Staphylococcus aureus Biofilms

Applied and Environmental Microbiology
Akshay K HarapanahalliHenny C van der Mei

Abstract

The majority of human infections are caused by biofilms. The biofilm mode of growth enhances the pathogenicity of Staphylococcus spp. considerably, because once they adhere, staphylococci embed themselves in a protective, self-produced matrix of extracellular polymeric substances (EPSs). The aim of this study was to investigate the influence of forces of staphylococcal adhesion to different biomaterials on icaA (which regulates the production of EPS matrix components) and cidA (which is associated with cell lysis and extracellular DNA [eDNA] release) gene expression in Staphylococcus aureus biofilms. Experiments were performed with S. aureus ATCC 12600 and its isogenic mutant, S. aureus ATCC 12600 Δpbp4, deficient in peptidoglycan cross-linking. Deletion of pbp4 was associated with greater cell wall deformability, while it did not affect the planktonic growth rate, biofilm formation, cell surface hydrophobicity, or zeta potential of the strains. The adhesion forces of S. aureus ATCC 12600 were the strongest on polyethylene (4.9 ± 0.5 nN), intermediate on polymethylmethacrylate (3.1 ± 0.7 nN), and the weakest on stainless steel (1.3 ± 0.2 nN). The production of poly-N-acetylglucosamine, eDNA presence, and expression of icaA gene...Continue Reading

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Citations

Aug 28, 2015·PLoS Pathogens·Akshay K HarapanahalliHenk J Busscher
Oct 18, 2016·ACS Applied Materials & Interfaces·Ferdi HizalHenny C van der Mei
Aug 23, 2017·Natural Product Research·Guofeng DongJianming Cao
Jul 18, 2018·Critical Reviews in Microbiology·Cristina CattòFrancesca Cappitelli
Nov 27, 2019·FEMS Microbiology Reviews·Tom E P Kimkes, Matthias Heinemann
Jun 26, 2020·NPJ Biofilms and Microbiomes·Can WangYijin Ren
Aug 7, 2019·International Journal of Molecular Sciences·Cristina Cattò, Francesca Cappitelli
Dec 4, 2016·Biochemical Society Transactions·Daniel Henry Stones, Anne Marie Krachler
Oct 29, 2020·Clinical Oral Investigations·Torsten SterzenbachMatthias Hannig
Aug 1, 2021·Microbiological Research·Rohit Ruhal, Rashmi Kataria
May 25, 2018·ACS Nano·Gi Byoung HwangIvan P Parkin

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