Influence of adjuvants on immunity in rabbits vaccinated with infective larval somatic proteins of Trichostrongylus colubriformis

Veterinary Parasitology
H Wedrychowicz, B Bezubik

Abstract

Adult New Zealand rabbits were vaccinated at 2-week intervals with three doses of 100 micrograms of infective larval somatic proteins (L3SP) administered subcutaneously with Freund's complete adjuvant (FCA) or beryllium hydroxide and then challenged orally with 10,000 L3. Groups of rabbits immunized orally with three doses of 5000 or 2000 L3 served as vaccination controls. Intestinal worm burdens on Day 21 after challenge revealed that beryllium hydroxide effectively potentiates the protective immunogenicity of L3SP. The level of protection obtained using the beryllium adjuvant (94.8%) was nearly as high as that in rabbits immunized with three doses of 5000 L3 (99.8%). Rabbits vaccinated using FCA showed very poor immunity (29.5%). Local and systemic antibody levels detected by radioimmunoprecipitation tests using 125I-L3SP showed very little correlation with the degree of protection. The beryllium hydroxide-treated group demonstrated significantly higher bile IgA antibody levels than other experimental rabbits. FCA-treated rabbits developed a much higher serum precipitating antibody response, detectable using gel double diffusion tests, than the beryllium group. Also, mucosal IgA antibody levels detected on Day 21 after challe...Continue Reading

References

Feb 1, 1978·The Australian Journal of Experimental Biology and Medical Science·A W Cripps, T L Rothwell
May 1, 1989·International Journal for Parasitology·I J O'DonnellC W Ward
Dec 1, 1988·International Journal for Parasitology·P G Douch
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Apr 1, 1980·The Australian Journal of Experimental Biology and Medical Science·D B AdamsA W Cripps
Feb 1, 1989·Parasitology Today·R Bomford

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Citations

Jan 1, 1996·International Journal for Parasitology·B M WaglandT L Rothwell
Jun 17, 2000·Infection and Immunity·J Y LeeM D Rossman
Jun 1, 1992·Research in Immunology·E ClaassenW Boersma
Aug 25, 2000·The Journal of Parasitology·H Ziam, V S Pandey

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