PMID: 30141604Dec 1, 2016Paper

Influence of Age on the Susceptibility of Anopheles stephensi to Plasmodium berghei Infection

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Xiu-mei Song, Jing-wen Wang

Abstract

To investigate the ability of Anopheles stephensi at different ages to transmit Plasmodium berghei and elucidate the possible mechanisms. To study and compare the susceptability of A. stephensi of different ages to P. berghei, 4-day and 25-day female A. stephensi were fed with P. berghei infected BALB/c mouse blood with parasitaemia 4%-8%. At 8 days after infection, the mosquitoes were dissected and the number of intestinal Plasmodium oocysts was counted under microscope. Difference in the susceptability to Plasmodium infection was analyzed between the two age groups. To study the intestinal bacteria load in A. stephensi, LB plate culture was used to detect the intestinal bacteria in the mosquitoes of the two groups before infection, and real-time quantitative PCR (qPCR) was performed to check the culturable bacteria and the total bacteria load. The expression levels of major immune response effectors cecropin(CEC1, CEC3), defensin (DEF), gambicin (GAM), attacin (ATT), nitric oxide synthase (NOS), dual oxidase (DUOX), and thioester protein 1(TEP1) in 4-day and 25-day mosquitoes were determined by qPCR. At 8 days after infection, the median of oocyst number in 4-day mosquitoes was 139, which was nearly 46 times of that in 25-day...Continue Reading

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