Influence of amino acid starvation on guanosine 5'-diphosphate 3'-diphosphate basal-level synthesis in Escherichia coli.

Journal of Bacteriology
P A Lagosky, F N Chang

Abstract

We observed that the synthesis of basal-level guanosine 5'-diphosphate 3'-diphosphate (ppGpp) in both relA mutants and relA+ relC strains of Escherichia coli decreased in response to amino acid limitation and that this was accompanied by an increase in ribonucleic acid (RNA) synthesis. Addition of the required amino acid to starved cultures of relaxed bacteria resulted in the resumption of ppGpp synthesis and a concomitant decrease in RNA production. Our results indicate that relA mutants retain a stringent factor-independent ribosomal mechanism for basal-level ppGpp synthesis. They also suggest that in relA+ bacteria, stringent factor-mediated ppGpp synthesis and the production of basal-level ppGpp are mutually exclusive. These findings substantiate the hypothesis that there are two functionally discrete mechanisms for ppGpp synthesis in E. coli. Through these studies we have also obtained new evidence which indicates that ppGpp serves as a modulator of RNA synthesis during balanced growth as well as under conditions of nutritional downshift and starvation.

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Citations

May 27, 2004·The Journal of Biological Chemistry·Miryoung SongHyon E Choy
Jan 1, 2011·Microbial Informatics and Experimentation·Sónia CarneiroIsabel Rocha
May 4, 2016·Journal of Bacteriology·Chengzhe TianNamiko Mitarai
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Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Patrick P DennisHans Bremer
Sep 10, 2020·Frontiers in Microbiology·Beny Spira, Katia Ospino
Jan 1, 1983·Molecular & General Genetics : MGG·E R Mackow, F N Chang
Oct 17, 2019·Amino Acids·Galina V SmirnovaOleg N Oktyabrsky
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Mar 1, 1982·Journal of Bacteriology·W T Charnetzky, R R Brubaker
Jun 1, 1991·Microbiological Reviews·A G Marr

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