Influence of amino acids on hybridoma cell viability and antibody secretion.

Cytotechnology
V GeaugeyJ M Engasser

Abstract

It is generally accepted that the phase of cell decline observed in batch culture of mammalian cells is related to exhaustion of medium nutrients (principally glucose and glutamine) and/or to waste products accumulation. In the present paper, we have studied the influence of glutamine on the proliferation of mouse hybridoma cells. We showed that repeated addition of glutamine prolonged the life span of the culture and significantly increased the secretion of monoclonal antibody. Flow cytometry analysis suggests that this effect of glutamine is related to a delay in cell death rather than to a stimulation of proliferation.Addition of glutamine and glucose failed however to prevent the death of the culture. Determinations of amino acid consumption in glutamine-supplemented samples and experiments carried out with complementary sources of amino acids (e.g. tryptose phosphate) strongly suggest that amino acid supply is a critical factor governing cell growth and productivity.

References

Jan 1, 1977·Journal of Biological Standardization·A Mizrahi, A Avihoo
Feb 15, 1988·Biochemical and Biophysical Research Communications·A HasegawaT Mori
Nov 14, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J P Griffiths, S J Pirt
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R W Holley, J A Kiernan
Jul 1, 1982·Cell Biology International Reports·W L McKeehan
Sep 1, 1981·Journal of Cellular Physiology·A Zetterberg, W Engström

❮ Previous
Next ❯

Citations

Aug 20, 1993·Biotechnology and Bioengineering·S K OhM G Yap
Feb 1, 1991·Cytotechnology·A MartialA Marc
Jan 1, 1991·Cytotechnology·H BüntemeyerJ Lehmann
Jun 11, 1991·Cytotechnology·F R NayveH Kataoka
Jan 1, 1994·Cytotechnology·V LeelavatcharamasM al-Rubeai
Dec 5, 1990·Journal of Immunological Methods·D DuvalA Marcadet
Jan 13, 1995·Journal of Immunological Methods·R JaspertJ L'age-Stehr
Jun 1, 1991·Journal of Biotechnology·M al-RubeaiS Chalder
Jan 15, 1995·Journal of Biotechnology·M G Solis-RecendezM Maugras
May 31, 1994·Journal of Biotechnology·F R NayveH Kataoka
Dec 11, 2002·Biotechnology and Bioengineering·Joel R CharbonneauEric R Gauthier
Mar 15, 1993·Biotechnology and Bioengineering·M V Cattaneo, J H Luong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.