Influence of angioscopic vein graft preparation on development of neointimal hyperplasia in an organ culture model of human saphenous vein

Journal of Endovascular Surgery : the Official Journal of the International Society for Endovascular Surgery
Y G WilsonG D Angelini

Abstract

Angioscopy for in situ vein graft preparation has been criticized on the basis that the trauma of instrumentation may predispose to accelerated intimal hyperplasia, jeopardizing patency rates following infrainguinal revascularization. The aim of this study was to assess the effects of angioscopic preparation on endothelial integrity and smooth muscle cell (SMC) behavior in an established organ culture model of human saphenous vein (HSV). HSV was harvested from 12 patients during bypass surgery before and after angioscopic preparation. Endothelial integrity was evaluated by immunohistochemical staining with JC-70 and scanning electron microscopy (SEM); remaining segments of pre- and postangioscopy vein were maintained in culture for 14 days in medium supplemented with 30% fetal calf serum. Viability was confirmed by measurement of tissue adenosine triphosphate on day 14 and thickness of the neointima was measured by computerized image analysis of histologic sections. Monoclonal antibodies to proliferating cell nuclear antigen (PCNA) were used as an immunohistochemical marker for proliferating SMCs. There was a significant reduction in the percentage staining by JC-70 (71.3% versus 20.4%) in pre- versus postangioscopy vein (p = 0...Continue Reading

References

Jan 1, 1992·Annals of Vascular Surgery·Y N HsiangM Weis
Jun 11, 1991·European Journal of Vascular Surgery·P A StonebridgeF W LoGerfo
Mar 1, 1991·Annals of Vascular Surgery·P A StonebridgeF W LoGerfo
Jul 31, 1987·The American Journal of Cardiology·J H ChesebroV Fuster
Jun 1, 1987·Journal of Vascular Surgery·M HashizumeT Matsumoto
Feb 1, 1966·Analytical Biochemistry·S AddankiP D Rearick
Oct 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M Hsu, E Soban
Aug 1, 1994·American Journal of Surgery·T R HarwardJ M Seeger
Apr 1, 1994·Journal of Vascular Surgery·K E AllenN J London
Jun 1, 1994·Journal of Vascular Surgery·D G ClairM C Donaldson
Jan 1, 1996·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·Y G WilsonP M Lamont

❮ Previous
Next ❯

Citations

Sep 5, 1998·The Surgical Clinics of North America·J V White, I Eid
Jan 1, 1977·Annales Chirurgiae Et Gynaecologiae·M Pakkanen
May 19, 2001·Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists·T YamakiK Sasaki
Jul 19, 2005·Journal of Biomechanics·Rebecca J GusicKeith J Gooch
Apr 5, 2012·Ultrastructural Pathology·S ValenteG Pasquinelli
Nov 18, 2008·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·T YamakiT Kono
Feb 28, 2002·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Takashi YamakiKenji Sasaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery
Y G WilsonG D Angelini
European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery
J Y JeremyG D Angelini
© 2021 Meta ULC. All rights reserved