Influence of antibiotic treatment on the detection of S. aureus in whole blood following pathogen enrichment

BMC Microbiology
Matthias PileckyViktoria Weber

Abstract

Early pathogen detection and identification are crucial for an effective and targeted antibiotic therapy in patients suffering from blood stream infection. Molecular diagnostic methods can accelerate pathogen identification as compared to blood culture, but frequently suffer from the inhibition of polymerase chain reation (PCR) by sample matrix components, such as host DNA, anticoagulants, or plasma proteins. To overcome this limitation, molecular diagnostic methods commonly rely on pathogen enrichment by selective lysis of blood cells and pelleting of intact pathogens prior to analysis. Here, we investigated the impact of antibiotic treatment on the recovery of pathogen DNA using an established pathogen enrichment protocol. Based on the hypothesis that induction of bacterial cell wall disintegration following antibiotic administration leads to incomplete pelleting of pathogen DNA, S. aureus was grown in human whole blood with or without addition of cell wall active (vancomycin, piperacillin) or non cell wall active (ciprofloxacin, clindamycin) antibiotics at clinically relevant concentrations. Pathogen detection remained unaffected by non cell wall active antibiotics or even increased in the presence of cell wall active antibi...Continue Reading

References

Feb 7, 2001·Journal of Clinical Microbiology·W A Al-Soud, P Rådström
May 8, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C J GraceB Littenberg
Mar 30, 2002·Journal of Clinical Microbiology·Marta E GarcíaDomingo Gargallo-Viola
Nov 1, 2002·Journal of Clinical Microbiology·Kaisu Rantakokko-Jalava, Jari Jalava
Mar 5, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G A Pankey, L D Sabath
Jul 20, 2006·Pediatric Hematology and Oncology·V DjordjevicM Radojkovic
Jul 21, 2006·The Cochrane Database of Systematic Reviews·V RafalskyE Rjabkova
Jul 25, 2006·Critical Care : the Official Journal of the Critical Care Forum·Jose Garnacho-MonteroCarlos Ortiz-Leyba
Nov 17, 2006·Annals of the New York Academy of Sciences·Svetlana N TamkovichPavel P Laktionov
Aug 21, 2007·International Journal of Antimicrobial Agents·Florence Fenollar, Didier Raoult
Jul 9, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Gerd DöringAlexandra Heininger
Feb 7, 2009·Enfermedades infecciosas y microbiología clínica·José Miguel MolinaMiguel Gobernado
Jan 13, 2010·Clinical Microbiology Reviews·Nicasio ManciniMassimo Clementi
Jun 10, 2010·Journal of Hospital Medicine : an Official Publication of the Society of Hospital Medicine·Michael B RothbergPeter K Lindenauer
Aug 19, 2010·The Journal of Infectious Diseases·Lorena TuchscherrBettina Löffler
Jan 7, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Catherine LiuUNKNOWN Infectious Diseases Society of America
Apr 29, 2011·PloS One·Natalia MalachowaFrank R DeLeo
Apr 11, 2012·British Journal of Clinical Pharmacology·Naïm BouazzaJean-Marc Tréluyer
Apr 11, 2012·BMC Infectious Diseases·Ryan LenzKevin B Laupland
May 24, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K B LauplandUNKNOWN International Bacteremia Surveillance Collaborative
Jul 31, 2012·International Journal of Pharmaceutics·Subhankari Prasad ChakrabortySomenath Roy
Aug 29, 2012·Blood Transfusion = Trasfusione Del Sangue·Gian Maria D'AmiciLello Zolla
Nov 29, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Silvie BernatováFilip Růžička
Dec 18, 2013·Virulence·Florian B MayrDerek C Angus
Jun 29, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Dennis L Stevens Infectious Diseases Society of America
Feb 28, 2016·Current Topics in Microbiology and Immunology·Kirsten J KoymansJos A G van Strijp
Mar 31, 2016·Current Topics in Microbiology and Immunology·Monica MonacoAnnalisa Pantosti
Apr 6, 2016·The Journal of Infection·Carol E MuenksStephanie A Fritz
Jan 19, 2017·Critical Care Medicine·Andrew RhodesR Phillip Dellinger
May 16, 2017·International Journal of Antimicrobial Agents·Heleen AardemaJan G Zijlstra
Nov 9, 2017·Expert Opinion on Pharmacotherapy·Carlota GudiolJordi Carratalà
Dec 21, 2017·European Journal of Drug Metabolism and Pharmacokinetics·Fawzy Elbarbry
Dec 27, 2017·International Journal of Antimicrobial Agents·Elena CarraraMical Paul
Mar 6, 2018·Analytical and Bioanalytical Chemistry·Maja SidstedtPeter Rådström
Jun 8, 2018·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C S ScheerS-O Kuhn
Dec 12, 2018·Applied and Environmental Microbiology·Richard C AllenAlex R Hall
Dec 24, 2018·Diagnostic Microbiology and Infectious Disease·Matthias PileckyViktoria Weber

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Citations

Feb 7, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Silke HuberDorothea Orth-Höller

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Methods Mentioned

BETA
PCR
scanning electron microscopy

Software Mentioned

SigmaPlot
GraphPad Prism
Roche LightCycler96

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