PMID: 7023120Mar 1, 1981Paper

Influence of antibodies on the phagocytosis of Klebsiella pneumoniae by alveolar macrophages (author's transl)

Zentralblatt Für Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie
C Undeutsch, H Brunner

Abstract

Phagocytosis of Klebsiella pneumoniae by alveolar macrophages of guinea pigs was investigated. Lavage of guinea pig lungs yielded a cell suspension of approximately 85% alveolar macrophages. The remaining cells were predominantly lymphocytes and a few polymorphonuclear leucocytes. After incubation for 5 hours at 37 degrees C the macrophages adhered to the glass surface whereas leucocytes could be washed away. This resulted in a pure culture of macrophages containing 95-98% living cells. Specific antibodies to phenol-water extracts of K. pneumoniae were induced in rabbits. These antisera increased the rate of uptake of the bacteria by macrophages at least 35-fold as compared to macrophages without added antiserum. Preimmunization sera of rabbits also increased the uptake of bacteria by macrophages but by far less than hyperimmune sera. A very sensitive Enzyme-Immuno-Assay (ELISA) for detection of antibodies to the phenol-water extracts of K. pneumoniae was developed. Low levels of antibodies were demonstrated in the pre-immunization sera of rabbits by ELISA but not by the tube agglutination test. These low antibody titers could explain the increased uptake of the bacteria by alveolar macrophages in the presence of preimmunizatio...Continue Reading

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.