Influence of antigen organization on the development of lupus autoantibodies

Arthritis and Rheumatism
S FatenejadJ Craft

Abstract

To investigate the reason for grouping of antibodies against small nuclear RNP (snRNP) particles, which are major autoantigens in systemic lupus erythematosus (SLE). Mice were immunized with biochemically purified native snRNP particles or recombinant proteins, followed by assessment of antibody and T cell responses. Since mouse (self) snRNPs are not immunogenic in mice, a eukaryotic expression vector was constructed to induce high-level expression of the human U1 snRNP-associated A protein in murine cells. Native chimeric (mouse/human) snRNP particles were used to immunize normal mice of both H-2k and H-2b backgrounds. We also disrupted the native snRNPs by digestion with ribonuclease and used this mixture of proteins to immunize mice. Immunization with native chimeric snRNPs resulted in the development of antibodies against a set of snRNP-associated proteins, a response which was accompanied by breakdown in T cell tolerance to mouse snRNPs in mice immunized with chimeric snRNPs. We also demonstrated that the ordered production of these antibodies was due to the fact that snRNP-associated proteins are grouped together in snRNP particles, since disruption of the particles resulted in development of antibodies in a random order,...Continue Reading

References

Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·P Lake, N A Mitchison
Feb 9, 1978·Nature·L L LanierP J Wettstein
Feb 1, 1978·The Journal of Experimental Medicine·R A EisenbergF J Dixon
Jan 1, 1991·Annual Review of Immunology·R M Steinman
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·G W Zieve, R A Sauterer
May 3, 1990·Nature·R M ZinkernagelH Arnheiter
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C A PennellG Haughton
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Sep 1, 1987·The Journal of Clinical Investigation·R A EisenbergP L Cohen
Feb 1, 1985·The Journal of Clinical Investigation·W H ReevesH G Kunkel
Jun 1, 1969·The Journal of Experimental Medicine·K RajewskyN K Jerne
Jan 1, 1983·Methods in Enzymology·W C Merrick
Jan 1, 1984·Current Topics in Microbiology and Immunology·C A PennellG Haughton
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E A LernerJ A Steitz
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·F TopferJ McCluskey
Jul 1, 1994·The Journal of Clinical Investigation·R W BurlingameR L Rubin
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S FatenejadJ Craft
Aug 1, 1993·Scandinavian Journal of Immunology·V HurezM D Kazatchkine
Apr 1, 1993·The Journal of Clinical Investigation·R W BurlingameA N Theofilopoulos
May 1, 1993·The Journal of Experimental Medicine·C MohanS K Datta
Oct 1, 1996·Clinical and Experimental Immunology·S Fatenejad, J Craft
Aug 1, 1997·Arthritis and Rheumatism·J Craft, S Fatenejad

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Citations

Sep 11, 2003·Autoimmunity Reviews·Gary W Zieve, Permanan R Khusial
Nov 20, 1998·The Journal of Clinical Investigation·R R SinghF M Ebling
Aug 13, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Umesh S DeshmukhShu Man Fu
Aug 9, 2005·Rheumatic Diseases Clinics of North America·Eric L Greidinger, Robert W Hoffman
Dec 23, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M C BosettoR M B Cicarelli
Jul 13, 2002·Arthritis and Rheumatism·Fanny Monneaux, Sylviane Muller
Jul 6, 2001·Scandinavian Journal of Immunology·F Monneaux, S Muller
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·U S DeshmukhS M Fu
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Micah T McClainJudith A James
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mihiri De Silva-UdawattaRobert W Hoffman
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Umesh S DeshmukhShu Man Fu
Mar 24, 2011·Expert Review of Clinical Immunology·Graham P WrightHans J Stauss
Sep 16, 2000·Current Opinion in Rheumatology·R W Hoffman, E L Greidinger

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