Influence of antigens and adjuvants on the production of gamma-interferon and antibody by ovine lymphocytes

Immunology and Cell Biology
D L EmeryP R Wood

Abstract

The production of gamma-interferon (gamma-IFN) and antigen-specific immunoglobulin isotypes was monitored in sheep given primary and secondary inocula of protein and polysaccharide antigens with or without adjuvants. In efferent lymph from prefemoral nodes draining the site of inoculation, gamma-IFN levels increased within 24 h after injection of adjuvants Quill A, dextran sulfate (DXS) and 6 days after oil adjuvants in saline. The increased synthesis of gamma-IFN was prolonged by 1-2 days by the presence of adjuvanted antigen, but the major stimulus to gamma-IFN production was provided by the adjuvant. Alhydrogel (AH) did not initiate gamma-IFN synthesis. Following a second inocula of antigen in saline, increased levels of gamma-IFN were detected in efferent lymph after 3-4 h, and persisted for at least 2 days. Following cultivation of leucocytes from immunized sheep with specific antigen in vitro, strong positive correlations between levels of interleukin (IL) IL-2, gamma-IFN, proliferative responses and antibody titres were observed. However, after analysis of the antigen-specific isotypes of immunoglobulin (Ig) there was no correlation between the production of gamma-IFN and particular Ig isotypes. Together with findings th...Continue Reading

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References

Jun 1, 1989·Veterinary Immunology and Immunopathology·G EntricanM Norval
Jul 1, 1988·European Journal of Immunology·E MaggiS Romagnani
Jun 1, 1987·Immunological Reviews·S SandvigU Andersson
Feb 1, 1988·Veterinary Immunology and Immunopathology·K J Beh
Jan 1, 1986·International Archives of Allergy and Applied Immunology·R M BarnesP M Johnson
Jan 1, 1987·The Journal of Experimental Medicine·J P CoutelierJ Van Snick
Oct 1, 1984·Veterinary Immunology and Immunopathology·G Oldham, L Williams
May 1, 1984·Cellular Immunology·L S English, M Whitehurst
Feb 1, 1989·Parasitology Today·R Bomford

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Citations

Nov 5, 1992·Journal of Immunological Methods·S L JonesA J Radford
Jun 1, 1992·Veterinary Immunology and Immunopathology·G EntricanD M Haig
Apr 1, 1994·Veterinary Immunology and Immunopathology·V M BowlesM R Brandon
May 1, 1995·The British Veterinary Journal·D P Jasmer, T C McGuire
Jun 5, 2003·International Journal for Parasitology·Manmohan Singh, Derek T O'Hagan
Sep 29, 1999·International Journal for Parasitology·D L EmeryT Bendixsen
Jul 10, 1998·Veterinary Immunology and Immunopathology·J S RothelP R Wood
Jun 6, 2000·Advanced Drug Delivery Reviews·J C CoxI G Barr
Sep 22, 2001·Biomolecular Engineering·D T O'HaganM Singh
Feb 19, 1998·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·D C Derosa, L M Sordillo
Oct 17, 2002·Annals of the New York Academy of Sciences·N GunterP Totte
Jun 29, 2013·Veterinary Immunology and Immunopathology·Anita GamvrellisJean-Pierre Y Scheerlinck
Jul 21, 2001·Tuberculosis·P R Wood, S L Jones
Mar 13, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Katrina L BoswardRichard J Whittington

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