Influence of AQP1 on cell adhesion, migration, and tumor sphere formation in malignant pleural mesothelioma is substratum- and histological-type dependent

American Journal of Physiology. Lung Cellular and Molecular Physiology
Rajesh M JagirdarSotirios G Zarogiannis

Abstract

Malignant pleural mesothelioma (MPM) is an aggressive cancer. MPM cells express aquaporin-1 (AQP1) that in other cancers has been shown to participate in the tumor metastasis processes. However, in MPM patients AQP1 overexpression is an independent prognostic factor favoring survival. In this study we aimed at evaluating the role of AQP1 in cell adhesion, migration, and tumor sphere formation in nonmalignant mesothelial cells (MeT-5A) and in epithelioid (M14K) and sarcomatoid (ZL34) MPM cell lines. We used fibronectin (FN) or homologous cell-derived extracellular martrix (ECM) substratum to investigate the role of AQP1 in these experimental phenotypes, inhibiting AQP1 by 10(-5) M mercury chloride (MC). Deposited ECM during cell culture exhibited significant concentration differences among cell types. ZL34 cell adhesion was significantly higher than MeT-5A or M14K cells on FN and ECM. MeT-5A and M14K cell adhesion on FN was sensitive to AQP1 inhibition, whereas AQP1 inhibition on ECM was limited to M14K cells. Wound healing in ZL34 cells was significantly higher than MeT-5A and M14K cells on FN and ECM. AQP1 inhibition significantly lowered cell migration in ZL34 cells on FN and ECM. Sphere formation was not dependent on FN or E...Continue Reading

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Citations

Jul 5, 2016·International Journal of Molecular Sciences·Jack DrimlSonja Klebe
Feb 12, 2013·The American Journal of Pathology·Brooke T MossmanRaffit Hassan
Mar 3, 2018·International Journal of Molecular Sciences·Giuseppe AngelicoVenerando Rapisarda
Mar 31, 2019·Pathology Oncology Research : POR·Mahdieh-Sadat Moosavi, Yalda Elham
Aug 12, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xin YunLarissa A Shimoda
Mar 23, 2019·Disease Models & Mechanisms·Jorge Del-PozoMichael Cheeseman
Dec 19, 2020·Clinical and Experimental Pharmacology & Physiology·Rajesh M JagirdarSotirios G Zarogiannis
Mar 2, 2021·Frontiers in Cell and Developmental Biology·Zihe HuoStephanie J Gros

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