PMID: 22344334Feb 22, 2012Paper

Influence of artificial saliva in biofilm formation of Candida albicans in vitro

Brazilian Oral Research
Michelle Peneluppi SilvaA O C Jorge

Abstract

Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 °C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 106 cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 °C for 5 days, the discs were washed in 2 mL ...Continue Reading

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Citations

May 2, 2015·The Journal of Advanced Prosthodontics·Burcin OnculFunda Dogruman Al
Nov 5, 2014·Jundishapur Journal of Microbiology·Ali Zarei MahmoudabadiNeda Kiasat
Feb 1, 2017·Molecular Medicine Reports·Sarra SebaaPhilippe Courtois
Mar 30, 2013·Brazilian Oral Research·Adriana Lígia de CastilhoIvana Barbosa Suffredini
Mar 25, 2019·International Journal of Molecular Sciences·Marcin MagaczWirginia Krzyściak

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