Abstract
Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 °C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 106 cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 °C for 5 days, the discs were washed in 2 mL ...Continue Reading
References
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·I H Valdez, P C Fox
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M J Levine
Aug 6, 1999·Oral Oncology·J B EpsteinP Stevenson-Moore
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D R RadfordJ D Walter
Jun 20, 2000·Palliative Medicine·A N Davies
Jan 13, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·S R PorterA M Hegarty
Feb 24, 2005·Journal of Dentistry·Michael W J DoddsChih-Ko Yeh
Apr 20, 2006·Archives of Oral Biology·Zaw M TheinLakshman P Samaranayake
May 9, 2006·Gerodontology·C Pesci-BardonI Madinier
Jan 20, 2007·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Piet DirixWalter Van den Bogaert
Feb 20, 2007·Oral Diseases·M-S ParkH-S Kho
Apr 9, 2008·Archives of Oral Biology·Tatiana Pereira-CenciWim Crielaard
Jul 22, 2008·International Journal of Oral and Maxillofacial Surgery·D-J OhH-S Kho
Dec 17, 2008·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Raymond RowanKevin Kavanagh
Jun 6, 2009·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Sebastian HahnelRalf Bürgers
Jul 30, 2009·Odontology·Joel J NapeñasPhilip C Fox
Aug 12, 2009·Eukaryotic Cell·Read Pukkila-WorleyEleftherios Mylonakis
Sep 1, 2009·Eukaryotic Cell·Lucja M JaroszBastiaan P Krom
Jan 5, 2010·Archives of Oral Biology·Moon-Soo ParkHong-Seop Kho
Mar 18, 2010·Oral Diseases·M-S ParkH-S Kho
Mar 23, 2010·Archives of Oral Biology·Sebastian HahnelRalf Bürgers
Apr 20, 2010·Current Microbiology·Sónia SilvaJoana Azeredo
Jun 1, 2010·Mycopathologia·Ranjith RajendranGordon Ramage
May 6, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C S-Y LimP P Chong
May 7, 2011·Journal of Oral Microbiology·David Williams, Michael Lewis
Jul 7, 2011·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·Pelin GüneriMustafa Ateş
Citations
Aug 25, 2015·Journal of Medical Microbiology·Paula Assis QueirozMelyssa Negri
May 2, 2015·The Journal of Advanced Prosthodontics·Burcin OnculFunda Dogruman Al
Nov 5, 2014·Jundishapur Journal of Microbiology·Ali Zarei MahmoudabadiNeda Kiasat
Feb 1, 2017·Molecular Medicine Reports·Sarra SebaaPhilippe Courtois
Mar 30, 2013·Brazilian Oral Research·Adriana Lígia de CastilhoIvana Barbosa Suffredini
Mar 25, 2019·International Journal of Molecular Sciences·Marcin MagaczWirginia Krzyściak