Influence of authentic nitric oxide on basal cytosolic [Ca2+] and Ca2+ release from internal stores in human platelets

British Journal of Pharmacology
K H Le Quan SangM A Devynck

Abstract

1. Nitric oxide (NO) donors inhibit platelet function and Ca2+ mobilization evoked by different agonists. This led us to investigate the direct effects of authentic NO on basal cytosolic Ca2+ concentration ([Ca2+]i) and on Ca2+ mobilization induced by thrombin or by two inhibitors of intracellular Ca(2+)-ATPases, thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone (t-BuBHQ). 2. Cytosolic Ca2+ concentration was evaluated with Fura-2, in the absence of Ca2+ influx. Addition of 5 microM NO increased by 48% the basal cytosolic [Ca2+] of resting human platelets whereas a lower concentration (0.1 microM) did not induce significant modifications. This NO-induced Ca2+ increase was inversely correlated with the basal level of cytosolic [Ca2+]. 3. NO pretreatment for 30 to 120 s decreased by 42 to 57% the transient [Ca2+]i peak evoked by 0.10 u ml-1 thrombin and strongly attenuated the initial rate of [Ca2+]i rise induced by 1 microM thapsigargin or by 20 microM t-BuBHQ. The two components of the thapsigargin response, the Ca2+ release due to inhibition of Ca2+ pumps and the thromboxane A2-dependent self-amplification mechanism, were inhibited by NO. The observation that a subsequent stimulation was still capable of eliciting Ca2+ re...Continue Reading

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Citations

Jan 9, 2001·Journal of Cellular Physiology·J X ChenB Meyrick
Nov 11, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·T OdaN Mukaida
Feb 15, 2008·Journal of Biochemical and Molecular Toxicology·Carlo A PalmeriniGiuseppe Arienti
Dec 14, 1999·Cephalalgia : an International Journal of Headache·G D'Andrea
Dec 14, 1999·Cephalalgia : an International Journal of Headache·P SarchielliV Gallai
Jul 29, 1998·Japanese Journal of Pharmacology·D M Helmeste, S W Tang
Dec 18, 2001·Journal of Applied Physiology·Daniel C HattonDavid A McCarron
Aug 15, 2001·American Journal of Physiology. Cell Physiology·C L BowmanJ W Lohr
Nov 9, 2010·Thrombosis Research·Peter F Blackmore

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