Influence of Bcl-2 overexpression on Na+/K(+)-ATPase pump activity: correlation with radiation-induced programmed cell death

Journal of Cellular Physiology
M Gilbert, S J Knox

Abstract

Bcl-2 overexpression in transfected PW cells is associated with inhibition of radiation-induced programmed cell death (PCD). We have previously reported that there is a relationship between inhibition of radiation-induced PCD and membrane hyperpolarization in these cells. In this article, we report that Na+/ K(+)-ATPase pump activity, as measured by the uptake of Rubidium-86 (86Rb+), is significantly higher in Bcl-2 overexpressing PW cells than in control PW cells, and that pump activity following irradiation with doses > or = 500 cGy was reduced to a lesser extent in the Bcl-2 transfectants than in the control cells. When PW-Bcl-2 cells were incubated with a dose of ouabain (1 microM) that decreased pump activity significantly, but did not induce PCD, the previously reported protection from radiation-induced PCD associated with overexpression of Bcl-2 no longer existed. In order to demonstrate that reactive oxygen species (ROS) affected Na+/ K(+)-ATPase pump activity, cells were incubated with N-acetyl cysteine (NAC) prior to irradiation, or treated with the ROS generating drug buthionine sulphoxamine (BSO). 86Rb+ uptake was significantly higher in irradiated cells incubated with NAC compared to cells irradiated in the absence...Continue Reading

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Citations

May 11, 2006·The Journal of Membrane Biology·R FrancoJ A Cidlowski
Apr 28, 2010·Apoptosis : an International Journal on Programmed Cell Death·Mihalis I PanayiotidisJohn A Cidlowski
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Jan 14, 2009·Seminars in Cancer Biology·Nathan SusnowDavid M Hockenbery
Jul 22, 2008·International Journal of Radiation Oncology, Biology, Physics·Takamitsu HaraTomio Inoue
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Apr 25, 2006·Free Radical Biology & Medicine·Andrei L KleschyovThomas Munzel
May 26, 2007·Biochimica Et Biophysica Acta·Andrey KulikovGeorgios Scheiner-Bobis
May 30, 2014·International Journal of Toxicology·M AbhilashR Harikumaran Nair
Oct 28, 2019·International Journal of Molecular Sciences·Marco CalvarusoLuigi Minafra

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