Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging

Optics Express
Sophie BrasseletS Brasselet

Abstract

We analyze the influence of the anisotropy of molecular and biological samples on polarization resolved nonlinear microscopy imaging. We show in particular the detrimental influence of birefringence on Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (TPEF) polarization resolved microscopy imaging, which, if not accounted for, can lead to an erroneous determination of the sample properties and thus to a misinterpretation of the read-out information. We propose a method to measure this birefringence and account for this effect in nonlinear polarization resolved experiments.

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Citations

Dec 19, 2012·Annals of Biomedical Engineering·Daniela Fátima Teixeira SilvaMartha Simões Ribeiro
Dec 12, 2012·Journal of Biomedical Optics·Dora Aït-BelkacemSophie Brasselet
Oct 2, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexander GustDina Grohmann
Jun 3, 2011·Optics Letters·Philippe RéfrégierSophie Brasselet
Dec 18, 2010·Optics Letters·Etienne ShafferChristian Depeursinge
Nov 22, 2011·Methods : a Companion to Methods in Enzymology·Levi M Haupert, Garth J Simpson
Jul 31, 2013·Methods : a Companion to Methods in Enzymology·Nirmal MazumderFu-Jen Kao
Oct 14, 2010·Optics Express·Ivan GusachenkoMarie-Claire Schanne-Klein
May 15, 2015·Optics Express·Hilton B de AguiarSophie Brasselet
Jun 5, 2014·Biophysical Journal·Patrick FerrandSophie Brasselet
Mar 12, 2013·Biophysical Journal·Sotiris PsilodimitrakopoulosPablo Loza-Alvarez
Jul 25, 2012·Biophysical Journal·Ivan GusachenkoMarie-Claire Schanne-Klein
Nov 16, 2010·The Analyst·Jeremy T MaddenGarth J Simpson
Sep 8, 2017·Science Advances·Hilton B de AguiarSophie Brasselet
Jun 14, 2013·Biomedical Optics Express·H LeeS-W Chu
Apr 9, 2019·Journal of the Royal Society, Interface·Jessica C MansfieldSophie Brasselet
Oct 2, 2012·Biomedical Optics Express·Raghu AmbekarKimani C Toussaint
Jan 19, 2012·Biomedical Optics Express·Gaël LatourMarie-Claire Schanne-Klein
Oct 20, 2012·Biomedical Optics Express·Sotiris PsilodimitrakopoulosDavid Artigas
Sep 15, 2020·Biomedical Optics Express·Emily G PendletonLuke J Mortensen
Sep 26, 2012·Physical Review Letters·Alicja GaseckaSophie Brasselet
Apr 9, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Chao-Wei HungGuan-Yu Zhuo

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