Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium.

Archives of Oral Biology
Fernanda Gonçalves BassoCarlos Alberto de Souza Costa

Abstract

This study aimed to assess the influence of the bisphosphonates zoledronic acid and sodium alendronate on MMP-2 and MMP-9 synthesis and activity by gingival fibroblasts seeded onto titanium substrate. Titanium discs were placed in 24-well cell culture plates and gingival fibroblasts were seeded (1 × 105 cells/discs) on them using Dulbecco's Modified Eagle's Medium (DMEM) + 10 % fetal bovine serum (FBS) for 24 h. After this period, a fresh serum-free DMEM containing zoledronic acid or sodium alendronate at 0.5 μM, 1 μM or 5 μM was applied on the cells for an additional of 24 h. Serum-free DMEM and tumor necrosis factor alpha (TNF-α) were used as negative and positive controls, respectively. MMP-2 and MMP-9 synthesis and activity were determined by ELISA (Enzyme-Linked Immunosorbent Assay) and conventional/in situ zymography. Quantitative data were analyzed by one-way ANOVA and Tukey's tests (α = 0.05). The in situ zymography data were qualitatively described. Despite both bisphosphonates increased the MMPs synthesis, this effect was significant higher in zoledronic acid groups. MMPs activity resembled by gelatinolytic activity was also enhanced by sodium alendronate and zoledronic acid in a similar pattern. Zoledronic acid and s...Continue Reading

References

Jan 5, 2002·Journal of the National Cancer Institute·Jeffrey A NemethMichael L Cher
Mar 1, 2002·Biology of Reproduction·William A RickeMichael F Smith
May 9, 2003·Acta Dermato-venereologica·Takashi KobayashiHiroshi Shinkai
Mar 16, 2004·Clinical Oral Implants Research·Jian MaYrjö T Konttinen
Nov 10, 2004·Oral Diseases·T SorsaT Salo
Mar 19, 2009·Clinical Oral Investigations·C WalterT Ziebart
Aug 5, 2009·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Mark A ScheperTimothy F Meiller
Nov 12, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M A LawsonR G G Russell
Jan 15, 2010·Clinical Oral Implants Research·Po-Chun ChangWilliam V Giannobile
Mar 24, 2010·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Towy Sorel LazaroviciNoam Yarom
Dec 15, 2010·Archives of Oral Biology·Matthew J RavosaM Sharon Stack
May 11, 2011·Bone·R Graham G Russell
Nov 22, 2011·Molecular Aspects of Medicine·Diego SbardellaMassimo Coletta
Mar 30, 2013·Journal of Periodontology·Patricia Furtado GonçalvesLuciana Macchion Shaddox
Jul 16, 2013·Oral Diseases·G SapnaK Bagri-Manjrekar
Aug 7, 2013·Expert Reviews in Molecular Medicine·Tülay Yucel-Lindberg, Tove Båge
Mar 25, 2014·International Journal of Oral and Maxillofacial Surgery·T Nogueira PansaniC A de Souza Costa
May 8, 2014·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Fernanda Gonçalves BassoCarlos Alberto de Souza Costa
Jun 1, 2014·Dental Materials : Official Publication of the Academy of Dental Materials·Carlos A de Souza CostaAna Paula D Ribeiro
Oct 30, 2014·Clinical Oral Investigations·Marianna De ColliAmelia Cataldi
Oct 2, 2015·Clinical Oral Implants Research·Hom-Lay WangWilliam V Giannobile
May 28, 2016·Oral Diseases·P G de Barros SilvaApnn Alves
Oct 30, 2016·Archives of Oral Biology·Viviane Oliveira SeverinoSanivia Aparecida de Lima Pereira
Nov 12, 2016·Journal of Oral & Maxillofacial Research·Erhan Dursun, Tolga Fikret Tözüm
Feb 22, 2017·International Journal of Molecular Sciences·Cavalla FrancoHernández Marcela
Apr 18, 2017·Progress in Molecular Biology and Translational Science·Ning CuiRaouf A Khalil
Jul 9, 2017·Clinical Oral Investigations·Fernanda Gonçalves BassoCarlos Alberto de Souza Costa
Feb 20, 2018·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Yahya AçilJörg Wiltfang
Jan 17, 2019·British Journal of Clinical Pharmacology·Serge CremersR Graham G Russell
Apr 10, 2019·Archives of Oral Biology·Taisa Nogueira PansaniCarlos Alberto de Souza Costa
Oct 3, 2019·Brazilian Oral Research·Monica Grazieli CorrêaMarcio Zaffalon Casati
Jul 16, 2020·Brazilian Dental Journal·Fernanda G BassoCarlos Alberto de Souza Costa

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Citations

May 23, 2021·Archives of Oral Biology·Laís Medeiros CardosoFernanda Gonçalves Basso

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