Influence of Brain Stem on Axial and Hindlimb Spinal Locomotor Rhythm Generating Circuits of the Neonatal Mouse

Frontiers in Neuroscience
Céline Jean-Xavier, Marie-Claude Perreault

Abstract

The trunk plays a pivotal role in limbed locomotion. Yet, little is known about how the brain stem controls trunk activity during walking. In this study, we assessed the spatiotemporal activity patterns of axial and hindlimb motoneurons (MNs) during drug-induced fictive locomotor-like activity (LLA) in an isolated brain stem-spinal cord preparation of the neonatal mouse. We also evaluated the extent to which these activity patterns are affected by removal of brain stem. Recordings were made in the segments T7, L2, and L5 using calcium imaging from individual axial MNs in the medial motor column (MMC) and hindlimb MNs in lateral motor column (LMC). The MN activities were analyzed during both the rhythmic and the tonic components of LLA, the tonic component being used as a readout of generalized increase in excitability in spinal locomotor networks. The most salient effect of brain stem removal was an increase in locomotor rhythm frequency and a concomitant reduction in burst durations in both MMC and LMC MNs. The lack of effect on the tonic component of LLA indicated specificity of action during the rhythmic component. Cooling-induced silencing of the brain stem reproduced the increase in rhythm frequency and accompanying decrea...Continue Reading

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Citations

Oct 31, 2019·Journal of Neurophysiology·Brandon K LaPalloMarie-Claude Perreault
Jun 11, 2019·Current Opinion in Physiology·Kimberly J Dougherty, Ngoc T Ha

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Methods Mentioned

BETA
dissection

Software Mentioned

Clampfit
Metamorph
pClamp Clampfit
Spinalcore
GraphPad Prism

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