PMID: 8592154Feb 1, 1996Paper

Influence of C terminus on monoamine oxidase A and B catalytic activity

Journal of Neurochemistry
K ChenJ C Shih

Abstract

Monoamine oxidase (MAO) A and B play important roles in the metabolism of neurotransmitters and dietary amines. The domains important for enzyme specificities were studied by construction of chimeric MAOA/B enzymes. Exchange of the N-terminal 45 amino acids of MAOA with the N-terminal 36 residues of MAOB (chimeric enzymes B36A and A45B) resulted in the same substrate and inhibitor sensitivities as the wild-type MAOA or B. Thus, the N terminus may not be responsible for MAOA or B enzyme specificities. When MAOB C-terminal residues 393-520 were replaced with MAOA C-terminal residues 402-527 (chimeric B393A) catalytic activity was not detectable. Chimeric B393A consists of eight residues with different charges, three less proline residues (458, 476, and 490), and one additional proline at 518 compared with wild-type MAOB. These differences may have induced conformational changes and affected MAOB catalytic activity. Thus, the C terminus of MAOB is critical for maintaining MAOB in an active form. It is interesting that when the C terminus of MAOA was switched with MAOB (chimeric A402B), little effect was observed on MAOA catalytic activity. This new information is valuable for further studies of the structure and function relations...Continue Reading

Citations

May 24, 2001·The Journal of Biological Chemistry·I RebrinJ C Shih
Mar 24, 2004·Inflammopharmacology·Noriko YaekashiwaHiroyasu Kinemuchi
Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Chi Chiu WangChristoph Ufer
May 30, 1997·The Journal of Biological Chemistry·Y Tsugeno, A Ito
Apr 15, 1999·Annual Review of Neuroscience·J C ShihM J Ridd
Jul 22, 1998·Proteins·J Wouters, G Baudoux
Feb 28, 2002·The Journal of Biological Chemistry·Rani Maurice GehaJean Chen Shih
Feb 19, 2000·Biochemical and Biophysical Research Communications·J H RohH Kumagai
Jul 28, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Liulin WangNam-Joon Cho
Nov 26, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Haruyo SugimotoHiroyasu Kinemuchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.