Influence of calcium on Manduca sexta plasmatocyte spreading and network formation

Archives of Insect Biochemistry and Physiology
E WillottHung Q Tran

Abstract

Plasmatocytes are a class of insect hemocytes important in the cellular defense response. In some species, they are phagocytic, protecting the insect from smaller pathogens. In many insects, they work in concert with other hemocytes (particularly other plasmatocytes and granular cells) to form nodules and to encapsulate foreign material. To perform these functions, plasmatocytes attach to, spread on, and surround suitable targets. Because of their importance, because we had previously observed that prolonged incubation of hemocytes in solutions containing the divalent cation chelator ethylenediaminetetraacetic acid (EDTA) inhibited plasmatocyte spreading, and because of the importance of divalent cations in many immune-related functions, we investigated the effect of calcium and magnesium on spreading of plasmatocytes from fifth instar Manduca sexta larvae. On glass slides, plasmatocytes spread more quickly and elongated in Grace's medium containing 5 mM calcium, compared to calcium-free medium. In the presence of calcium, plasmatocyte adhesion, spreading, and network formation were not visibly different in magnesium-free and magnesium-containing Grace's medium. Using immunomicroscopy with a monoclonal antibody specific for pla...Continue Reading

References

Sep 1, 1971·Journal of Invertebrate Pathology·F D Brewer, S B Vinson
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·J S MillerD W Stanley-Samuelson
Jan 1, 1995·Annual Review of Entomology·M R Strand, L L Pech
Oct 1, 1994·Current Opinion in Cell Biology·P J GotwalsR O Hynes
Jan 1, 1993·Current Topics in Developmental Biology·T A Bunch, D L Brower
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek
Feb 14, 1998·European Journal of Biochemistry·R BettencourtI Faye
Jul 30, 1999·Developmental and Comparative Immunology·M W Johansson
Oct 26, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·D W StanleyJ S Miller
Oct 27, 1999·Archives of Insect Biochemistry and Physiology·M R Strand, K D Clark
Dec 3, 1999·European Journal of Biochemistry·R BettencourtI Faye
Aug 5, 2000·The Journal of Biological Chemistry·R C BowersR C Murphy
Nov 21, 2000·The International Journal of Biochemistry & Cell Biology·A A Maghazachi

❮ Previous
Next ❯

Citations

Aug 23, 2003·Behavioral Neuroscience·James F WillottLeona Gagnon
Oct 26, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Arash ZibaeeDavide Malagoli
Apr 15, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D O PinheiroF L Cônsoli
Sep 18, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Saleem AladailehDavid A Raftos
Apr 12, 2005·Archives of Insect Biochemistry and Physiology·Jon S Miller
Mar 26, 2003·Archives of Insect Biochemistry and Physiology·Yuichi NakaharaManabu Kamimura
Jun 20, 2015·Archives of Insect Biochemistry and Physiology·Natalia A KryukovaVictor V Glupov
Oct 19, 2011·Archives of Insect Biochemistry and Physiology·Koigoora SrikanthYonggyun Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.